TY - JOUR
T1 - Three types of amyloid protein precursor mRNA in human brain
T2 - Their differential expression in Alzheimer's disease
AU - Tanaka, Seigo
AU - Nakamura, Shigenobu
AU - Ueda, Kunihiro
AU - Kameyama, Masakuni
AU - Shiojiri, Satoshi
AU - Takahashi, Yasuyuki
AU - Kitaguchi, Nobuya
AU - Ito, Hirataka
N1 - Funding Information:
We thank Drs. M. Ogawa, T. Seko, K. Hara, R. Matsumoto and T. Kimura for providing us with brain samples, and Dr. I. Saito and Mr. S. Horiguchi for useful discussion. The work was partly supported by Grants-in-Aid and Special Grant for Clinical Investigation from the Ministry of Education, Science and Culture, Japan.
PY - 1988/12/15
Y1 - 1988/12/15
N2 - Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.
AB - Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.
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U2 - 10.1016/S0006-291X(88)80273-0
DO - 10.1016/S0006-291X(88)80273-0
M3 - Article
C2 - 3144271
AN - SCOPUS:0024235896
SN - 0006-291X
VL - 157
SP - 472
EP - 479
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -