Three types of amyloid protein precursor mRNA in human brain: Their differential expression in Alzheimer's disease

Seigo Tanaka; Shigenobu Nakamura; Kunihiro Ueda; Masakuni Kameyama; Satoshi Shiojiri; Yasuyuki Takahashi; Nobuya Kitaguchi; Hirataka Ito

doi:10.1016/S0006-291X(88)80273-0

Three types of amyloid protein precursor mRNA in human brain: Their differential expression in Alzheimer's disease

Seigo Tanaka
, Shigenobu Nakamura
, Kunihiro Ueda
, Masakuni Kameyama
, Satoshi Shiojiri
, Yasuyuki Takahashi
, Nobuya Kitaguchi
, Hirataka Ito

Research output: Contribution to journal › Article › peer-review

129 Citations (Scopus)

Abstract

Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.

Original language	English
Pages (from-to)	472-479
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	157
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(88)80273-0
Publication status	Published - 15-12-1988
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(88)80273-0

Cite this

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title = "Three types of amyloid protein precursor mRNA in human brain: Their differential expression in Alzheimer's disease",

abstract = "Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes {"}APP714{"} containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.",

author = "Seigo Tanaka and Shigenobu Nakamura and Kunihiro Ueda and Masakuni Kameyama and Satoshi Shiojiri and Yasuyuki Takahashi and Nobuya Kitaguchi and Hirataka Ito",

note = "Funding Information: We thank Drs. M. Ogawa, T. Seko, K. Hara, R. Matsumoto and T. Kimura for providing us with brain samples, and Dr. I. Saito and Mr. S. Horiguchi for useful discussion. The work was partly supported by Grants-in-Aid and Special Grant for Clinical Investigation from the Ministry of Education, Science and Culture, Japan.",

year = "1988",

month = dec,

day = "15",

doi = "10.1016/S0006-291X(88)80273-0",

language = "English",

volume = "157",

pages = "472--479",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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TY - JOUR

T1 - Three types of amyloid protein precursor mRNA in human brain

T2 - Their differential expression in Alzheimer's disease

AU - Tanaka, Seigo

AU - Nakamura, Shigenobu

AU - Ueda, Kunihiro

AU - Kameyama, Masakuni

AU - Shiojiri, Satoshi

AU - Takahashi, Yasuyuki

AU - Kitaguchi, Nobuya

AU - Ito, Hirataka

N1 - Funding Information: We thank Drs. M. Ogawa, T. Seko, K. Hara, R. Matsumoto and T. Kimura for providing us with brain samples, and Dr. I. Saito and Mr. S. Horiguchi for useful discussion. The work was partly supported by Grants-in-Aid and Special Grant for Clinical Investigation from the Ministry of Education, Science and Culture, Japan.

PY - 1988/12/15

Y1 - 1988/12/15

N2 - Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.

AB - Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease. These mRNAs encode APP695 consisting of 695 amino acids, APP751 harboring a 56 amino acid insert homologous to a Kunitz-type trypsin inhibitor inside APP695, and APP770 containing an additional 19 amino acid insert. Another possible APP mRNA which encodes "APP714" containing a 19 amino acid insert was not found in brain samples tested. Quantitative analysis revealed that, although the relative expression levels of the three mRNAs were variable among individuals, there was no remarkable change in expression of APP695 and APP751 mRNAs in Alzheimer's disease compared with control, but that APP770 mRNA level was elevated significantly in Alzheimer's disease.

UR - https://www.scopus.com/pages/publications/0024235896

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U2 - 10.1016/S0006-291X(88)80273-0

DO - 10.1016/S0006-291X(88)80273-0

M3 - Article

C2 - 3144271

AN - SCOPUS:0024235896

SN - 0006-291X

VL - 157

SP - 472

EP - 479

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Three types of amyloid protein precursor mRNA in human brain: Their differential expression in Alzheimer's disease

Abstract

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