Abstract
Tight junction (TJ)-like structures have been reported in Schwann cells, but their molecular composition and physiological function remain elusive. We found that claudin-19, a novel member of the claudin family (TJ adhesion molecules in epithelia), constituted these structures. Claudin-19-deficient mice were generated, and they exhibited behavioral abnormalities that could be attributed to peripheral nervous system deficits. Electrophysiological analyses showed that the claudin-19 deficiency affected the nerve conduction of peripheral myelinated fibers. Interestingly, the overall morphology of Schwann cells lacking claudin-19 expression appeared to be normal not only in the internodal region but also at the node of Ranvier, except that TJs completely disappeared, at least from the outer/inner mesaxons. These findings have indicated that, similar to epithelial cells, Schwann cells also bear claudin-based TJs, and they have also suggested that these TJs are not involved in the polarized morphogenesis but are involved in the electrophysiological "sealing" function of Schwann cells.
| Original language | English |
|---|---|
| Pages (from-to) | 527-538 |
| Number of pages | 12 |
| Journal | Journal of Cell Biology |
| Volume | 169 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 09-05-2005 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Cell Biology
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Tight junctions in Schwann cells of peripheral myelinated axons : A lesson from claudin-19-deficient mice. / Miyamoto, Tatsuo; Morita, Kazumasa; Takemoto, Daisuke; Takeuchi, Kosei; Kitano, Yuka; Miyakawa, Tsuyoshi; Nakayama, Kiyomi; Okamura, Yasushi; Sasaki, Hiroyuki; Miyachi, Yoshiki; Furuse, Mikio; Tsukita, Shoichiro.
In: Journal of Cell Biology, Vol. 169, No. 3, 09.05.2005, p. 527-538.Research output: Contribution to journal › Article
TY - JOUR
T1 - Tight junctions in Schwann cells of peripheral myelinated axons
T2 - A lesson from claudin-19-deficient mice
AU - Miyamoto, Tatsuo
AU - Morita, Kazumasa
AU - Takemoto, Daisuke
AU - Takeuchi, Kosei
AU - Kitano, Yuka
AU - Miyakawa, Tsuyoshi
AU - Nakayama, Kiyomi
AU - Okamura, Yasushi
AU - Sasaki, Hiroyuki
AU - Miyachi, Yoshiki
AU - Furuse, Mikio
AU - Tsukita, Shoichiro
PY - 2005/5/9
Y1 - 2005/5/9
N2 - Tight junction (TJ)-like structures have been reported in Schwann cells, but their molecular composition and physiological function remain elusive. We found that claudin-19, a novel member of the claudin family (TJ adhesion molecules in epithelia), constituted these structures. Claudin-19-deficient mice were generated, and they exhibited behavioral abnormalities that could be attributed to peripheral nervous system deficits. Electrophysiological analyses showed that the claudin-19 deficiency affected the nerve conduction of peripheral myelinated fibers. Interestingly, the overall morphology of Schwann cells lacking claudin-19 expression appeared to be normal not only in the internodal region but also at the node of Ranvier, except that TJs completely disappeared, at least from the outer/inner mesaxons. These findings have indicated that, similar to epithelial cells, Schwann cells also bear claudin-based TJs, and they have also suggested that these TJs are not involved in the polarized morphogenesis but are involved in the electrophysiological "sealing" function of Schwann cells.
AB - Tight junction (TJ)-like structures have been reported in Schwann cells, but their molecular composition and physiological function remain elusive. We found that claudin-19, a novel member of the claudin family (TJ adhesion molecules in epithelia), constituted these structures. Claudin-19-deficient mice were generated, and they exhibited behavioral abnormalities that could be attributed to peripheral nervous system deficits. Electrophysiological analyses showed that the claudin-19 deficiency affected the nerve conduction of peripheral myelinated fibers. Interestingly, the overall morphology of Schwann cells lacking claudin-19 expression appeared to be normal not only in the internodal region but also at the node of Ranvier, except that TJs completely disappeared, at least from the outer/inner mesaxons. These findings have indicated that, similar to epithelial cells, Schwann cells also bear claudin-based TJs, and they have also suggested that these TJs are not involved in the polarized morphogenesis but are involved in the electrophysiological "sealing" function of Schwann cells.
UR - http://www.scopus.com/inward/record.url?scp=21044437802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21044437802&partnerID=8YFLogxK
U2 - 10.1083/jcb.200501154
DO - 10.1083/jcb.200501154
M3 - Article
C2 - 15883201
AN - SCOPUS:21044437802
VL - 169
SP - 527
EP - 538
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 3
ER -