Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation

Kei Noguchi, Hiroyuki Tomita, Tomohiro Kanayama, Ayumi Niwa, Yuichiro Hatano, Masato Hoshi, Shigeyuki Sugie, Hideshi Okada, Masayuki Niwa, Akira Hara

Research output: Contribution to journalArticle

Abstract

Galectin-3 is a β-galactoside-binding lectin which is important in cell proliferation and apo-ptotic regulation. Recently, serum galectin-3 has been shown to have prognostic value as a biomarker in heart failure. Encephalomyocarditis virus (EMCV) can cause severe myocarditis, congestive heart failure and dilated cardiomyopathy as well as encephalitis in various animals including mice. The pathophysiological role of galectin-3 in acute myocarditis following viral infection is not fully understood. The goal of this study is to determine the cardiac localization and the time-course of galectin-3 expression in heart failure after viral inoculation with EMCV. At 12, 24, 48, 96 hours, 7 and 10 days after intraperitoneal EMCV inoculation, animals were examined histologically and analyzed for the expression of galectin-3 and Iba1. Galectin-3 was up-regulated in degenerated fibrotic lesions of cardiac tissues 96 hours after viral inoculation and were followed by myocardial fibrosis. At the same time, Iba1 positive macrophages were observed within the inflammatory sites. A time-course correlation between the number of galectin-3 positive cells and the cardiac area of degenerated fibrotic lesions was detected—serum galectin-3 increased at 96 hours and correlated well with the number of cardiac galectin-3 positive cells. Our results indicate that galectin-3 expression may be a useful biomarker of cardiac fibrotic degeneration in acute myocarditis following viral infection. In addition, measuring serum galectin-3 levels might be an early diagnostic method for detecting cardiac degeneration in acute myocarditis.

Original languageEnglish
Article numbere0210971
JournalPloS one
Volume14
Issue number1
DOIs
Publication statusPublished - 01-2019

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Encephalomyocarditis virus
Galectin 3
myocarditis
Myocarditis
Viruses
heart failure
Serum
lesions (animal)
biomarkers
galactosides
cardiomyopathy
encephalitis
fibrosis
infection
lectins
diagnostic techniques
Heart Failure
cell proliferation
animals
macrophages

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Noguchi, Kei ; Tomita, Hiroyuki ; Kanayama, Tomohiro ; Niwa, Ayumi ; Hatano, Yuichiro ; Hoshi, Masato ; Sugie, Shigeyuki ; Okada, Hideshi ; Niwa, Masayuki ; Hara, Akira. / Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation. In: PloS one. 2019 ; Vol. 14, No. 1.
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Noguchi, K, Tomita, H, Kanayama, T, Niwa, A, Hatano, Y, Hoshi, M, Sugie, S, Okada, H, Niwa, M & Hara, A 2019, 'Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation', PloS one, vol. 14, no. 1, e0210971. https://doi.org/10.1371/journal.pone.0210971

Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation. / Noguchi, Kei; Tomita, Hiroyuki; Kanayama, Tomohiro; Niwa, Ayumi; Hatano, Yuichiro; Hoshi, Masato; Sugie, Shigeyuki; Okada, Hideshi; Niwa, Masayuki; Hara, Akira.

In: PloS one, Vol. 14, No. 1, e0210971, 01.2019.

Research output: Contribution to journalArticle

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T1 - Time-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation

AU - Noguchi, Kei

AU - Tomita, Hiroyuki

AU - Kanayama, Tomohiro

AU - Niwa, Ayumi

AU - Hatano, Yuichiro

AU - Hoshi, Masato

AU - Sugie, Shigeyuki

AU - Okada, Hideshi

AU - Niwa, Masayuki

AU - Hara, Akira

PY - 2019/1

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