Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Kazuki Nakajima; Yasuo Oda; Mitsuhiro Kinoshita; Takashi Masuko; Kazuaki Kakehi

doi:10.1039/b202950b

Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Kazuki Nakajima, Yasuo Oda, Mitsuhiro Kinoshita, Takashi Masuko, Kazuaki Kakehi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We examined the formation of complexes between terbium ion (Tb³⁺) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb³⁺ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

Original language	English
Pages (from-to)	972-976
Number of pages	5
Journal	Analyst
Volume	127
Issue number	7
DOIs	https://doi.org/10.1039/b202950b
Publication status	Published - 2002

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/b202950b

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title = "Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination",

abstract = "We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.",

author = "Kazuki Nakajima and Yasuo Oda and Mitsuhiro Kinoshita and Takashi Masuko and Kazuaki Kakehi",

year = "2002",

doi = "10.1039/b202950b",

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T1 - Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

AU - Nakajima, Kazuki

AU - Oda, Yasuo

AU - Kinoshita, Mitsuhiro

AU - Masuko, Takashi

AU - Kakehi, Kazuaki

PY - 2002

Y1 - 2002

N2 - We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

AB - We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

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U2 - 10.1039/b202950b

DO - 10.1039/b202950b

M3 - Article

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AN - SCOPUS:0036325483

SN - 0003-2654

VL - 127

SP - 972

EP - 976

JO - Analyst

JF - Analyst

IS - 7

ER -

Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Abstract

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