Time-resolved fluorometric analysis of carbohydrates labeled with amino-aromatic compounds by reductive amination

Kazuki Nakajima, Yasuo Oda, Mitsuhiro Kinoshita, Takashi Masuko, Kazuaki Kakehi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We examined the formation of complexes between terbium ion (Tb3+) and carbohydrates labeled with aminobenzene compounds. Of the examined compounds, carbohydrates labeled with 4-aminosalicylic acid showed intense fluorescence with Tb3+ in the presence of cetylpyridinium chloride at pH 6.0. Calibration curves for maltose derivative showed good linearity between 5 pmol and at least 600 pmol, with good reproducibility. We applied the proposed technique to binding studies between manno-oligosaccharides and Concanavalin A.

Original languageEnglish
Pages (from-to)972-976
Number of pages5
JournalAnalyst
Volume127
Issue number7
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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