TY - JOUR
T1 - Tissue culture methods to study neurological disorders
T2 - Establishment of immortalized Schwann cells from murine disease models
AU - Watabe, Kazuhiko
AU - Sakamoto, Tsuyoshi
AU - Kawazoe, Yoko
AU - Michikawa, Makoto
AU - Miyamoto, Katsuichi
AU - Yamamura, Takashi
AU - Saya, Hideyuki
AU - Araki, Norie
PY - 2003/3
Y1 - 2003/3
N2 - Previously, the authors have established spontaneously immortalized cell lines from long-term cultures of normal adult mouse Schwann cells. Establishment of such Schwann cell lines derived from murine disease models may greatly facilitate studies of the cellular mechanisms of their peripheral nervous system lesions in the relevant diseases. Recently, the authors have established immortalized Schwann cell lines derived from Niemann-Pick disease type C mice (NPC; spm/spm) and globoid cell leukodystrophy mice (twitcher). In the present study, long-term cultures were maintained of Schwann cells derived from dorsal root ganglia and consecutive peripheral nerves of another NPC mouse (npcnih/npcnih, npcnih/+), myelin P0 protein-deficient mice (P0-/-, P0+/-) with their wild-type littermates (P0+/+), and neurofibromatosis type 1 gene (NF1)-deficient mice (Nf1Fcr/+) for 8-10 months, and immortalized cell lines from all these animals established spontaneously. These cell lines had spindle-shaped Schwann cell morphology and distinct Schwann cell phenotypes and retained genomic and biochemical abnormalities, sufficiently representing the in vivo pathological features of the mutant mice. These immortalized Schwann cell lines can be useful in studies of nervous system lesions in these mutant mice and relevant human disorders.
AB - Previously, the authors have established spontaneously immortalized cell lines from long-term cultures of normal adult mouse Schwann cells. Establishment of such Schwann cell lines derived from murine disease models may greatly facilitate studies of the cellular mechanisms of their peripheral nervous system lesions in the relevant diseases. Recently, the authors have established immortalized Schwann cell lines derived from Niemann-Pick disease type C mice (NPC; spm/spm) and globoid cell leukodystrophy mice (twitcher). In the present study, long-term cultures were maintained of Schwann cells derived from dorsal root ganglia and consecutive peripheral nerves of another NPC mouse (npcnih/npcnih, npcnih/+), myelin P0 protein-deficient mice (P0-/-, P0+/-) with their wild-type littermates (P0+/+), and neurofibromatosis type 1 gene (NF1)-deficient mice (Nf1Fcr/+) for 8-10 months, and immortalized cell lines from all these animals established spontaneously. These cell lines had spindle-shaped Schwann cell morphology and distinct Schwann cell phenotypes and retained genomic and biochemical abnormalities, sufficiently representing the in vivo pathological features of the mutant mice. These immortalized Schwann cell lines can be useful in studies of nervous system lesions in these mutant mice and relevant human disorders.
UR - http://www.scopus.com/inward/record.url?scp=0037354956&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037354956&partnerID=8YFLogxK
U2 - 10.1046/j.1440-1789.2003.00478.x
DO - 10.1046/j.1440-1789.2003.00478.x
M3 - Article
C2 - 12722929
AN - SCOPUS:0037354956
SN - 0919-6544
VL - 23
SP - 68
EP - 78
JO - Neuropathology
JF - Neuropathology
IS - 1
ER -