Tissue culture of Aloe arborescens Miller var. natalensis Berger

Kaoru Kawai, Hidehiko Beppu, Takaaki Koike, Keisuke Fujita, Tohru Marunouchi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We examined the culture conditions for callus induction in the tissue of Aloe arborescens. When incubated in the dark at 25°C, for about 30 days callus was induced. The frequency of callus formation was in the order of superior stalk > middle stalk > inferior stalk. No callus was formed in the leaf. When the Murashige–Skoog (MS) basal agar medium was supplemented with 3% sucrose, 0.1–0.5 μM kinetin (a plant growth regulator) and 10–50 μM alpha‐naphthalenacetic acid, callus formation occurred at an incidence of 20–52% (mean: 39%). When the MS basal agar medium was supplemented with 3% glucose and 10% Alpha Modification of Eagle's Medium instead of 3% sucrose, callus formation was promoted, but the protein and aloin concentrations, and carboxypeptidase activity in the Aloe callus decreased more than those observed in the sucrose‐containing medium.

Original languageEnglish
Pages (from-to)S5-S10
JournalPhytotherapy Research
Volume7
Issue number7
DOIs
Publication statusPublished - 01-01-1993

All Science Journal Classification (ASJC) codes

  • Pharmacology

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