Tissue factor expression demonstrates severe sinusoidal endothelial cell damage during rejection after living-donor liver transplantation

Masanobu Usui, Naohisa Kuriyama, Masashi Kisawada, Takashi Hamada, Shugo Mizuno, Hiroyuki Sakurai, Masami Tabata, Hiroshi Imai, Kohji Okamoto, Shinji Uemoto, Shuji Isaji

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Background/Purpose: Since it is well known that endothelial cells may be important targets during rejection after living-donor liver transplantation, in this study we investigated liver sinusoidal endothelial cell (SEC) damage during rejection by focusing on thrombomodulin (TM) and hyaluronic acid (HA) as plasma markers of SEC damage. We also examined tissue factor (TF) expression in SECs, because damage to endothelial cells leads to immediate activation of the coagulation system, with the damage being triggered mainly by TF. Methods: Living-donor liver transplantation was performed at Mie University Hospital between March 2002 and December 2007; there were 8 patients with rejection (4 with acute cellular rejection and 4 with chronic rejection) and 32 patients without rejection. Liver biopsy tissue was immunostained with an anti-TF antibody, and assessed for SEC damage. In addition, total RNA was extracted from liver biopsy tissue and tested for TF mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR). Results: The plasma TM level was significantlyhigher in the rejection group than in the non-rejection group. TF expression was observed in SECs, in infiltrating inflammatory cells, and in the vascular endothelium in the rejection group. TF mRNA expression was significantly higher in the rejection group than in the non-rejection group. Conclusions: We demonstrated that TF expression revealed severe SEC damage in grafted liver during both acute cellular rejection and chronic rejection.

Original languageEnglish
Pages (from-to)513-520
Number of pages8
JournalJournal of Hepato-Biliary-Pancreatic Surgery
Volume16
Issue number4
DOIs
Publication statusPublished - 06-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Hepatology

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