Tissue-specific expression of three types of β-protein precursor mRNA: Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain

Seigo Tanaka, Satoshi Shiojiri, Yasuyuki Takahashi, Nobuya Kitaguchi, Hirataka Ito, Masakuni Kameyama, Jun Kimura, Shigenobu Nakamura, Kunihiro Ueda

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.

Original languageEnglish
Pages (from-to)1406-1414
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number3
DOIs
Publication statusPublished - 30-12-1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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