TY - JOUR
T1 - Tissue-specific expression of three types of β-protein precursor mRNA
T2 - Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain
AU - Tanaka, Seigo
AU - Shiojiri, Satoshi
AU - Takahashi, Yasuyuki
AU - Kitaguchi, Nobuya
AU - Ito, Hirataka
AU - Kameyama, Masakuni
AU - Kimura, Jun
AU - Nakamura, Shigenobu
AU - Ueda, Kunihiro
N1 - Funding Information:
Reserve University) for informing us of their paper in press. This work was partly supported by Grants-in-Aid and Special Grant for Clinical Investigation from the Ministry of Education, Science and Culture, Japan, and grant from Sasagawa Health Science Foundation.
PY - 1989/12/30
Y1 - 1989/12/30
N2 - Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.
AB - Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.
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U2 - 10.1016/0006-291X(89)92760-5
DO - 10.1016/0006-291X(89)92760-5
M3 - Article
C2 - 2514687
AN - SCOPUS:0024847273
SN - 0006-291X
VL - 165
SP - 1406
EP - 1414
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -