Tissue-specific expression of three types of β-protein precursor mRNA: Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain

Seigo Tanaka, Satoshi Shiojiri, Yasuyuki Takahashi, Nobuya Kitaguchi, Hirataka Ito, Masakuni Kameyama, Jun Kimura, Shigenobu Nakamura, Kunihiro Ueda

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.

Original languageEnglish
Pages (from-to)1406-1414
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume165
Issue number3
DOIs
Publication statusPublished - 30-12-1989

Fingerprint

Protein Precursors
Protease Inhibitors
Brain
Alzheimer Disease
Tissue
Messenger RNA
Amyloid beta-Protein Precursor
Amyloidogenic Proteins
Biosynthesis
Ribonucleases
Amyloid
Cerebral Cortex
Assays
Degradation
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tanaka, Seigo ; Shiojiri, Satoshi ; Takahashi, Yasuyuki ; Kitaguchi, Nobuya ; Ito, Hirataka ; Kameyama, Masakuni ; Kimura, Jun ; Nakamura, Shigenobu ; Ueda, Kunihiro. / Tissue-specific expression of three types of β-protein precursor mRNA : Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 165, No. 3. pp. 1406-1414.
@article{788a16732bb94d97823889e6cf1fdce3,
title = "Tissue-specific expression of three types of β-protein precursor mRNA: Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain",
abstract = "Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.",
author = "Seigo Tanaka and Satoshi Shiojiri and Yasuyuki Takahashi and Nobuya Kitaguchi and Hirataka Ito and Masakuni Kameyama and Jun Kimura and Shigenobu Nakamura and Kunihiro Ueda",
year = "1989",
month = "12",
day = "30",
doi = "10.1016/0006-291X(89)92760-5",
language = "English",
volume = "165",
pages = "1406--1414",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

Tissue-specific expression of three types of β-protein precursor mRNA : Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain. / Tanaka, Seigo; Shiojiri, Satoshi; Takahashi, Yasuyuki; Kitaguchi, Nobuya; Ito, Hirataka; Kameyama, Masakuni; Kimura, Jun; Nakamura, Shigenobu; Ueda, Kunihiro.

In: Biochemical and Biophysical Research Communications, Vol. 165, No. 3, 30.12.1989, p. 1406-1414.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tissue-specific expression of three types of β-protein precursor mRNA

T2 - Enhancement of protease inhibitor-harboring types in Alzheimer's disease brain

AU - Tanaka, Seigo

AU - Shiojiri, Satoshi

AU - Takahashi, Yasuyuki

AU - Kitaguchi, Nobuya

AU - Ito, Hirataka

AU - Kameyama, Masakuni

AU - Kimura, Jun

AU - Nakamura, Shigenobu

AU - Ueda, Kunihiro

PY - 1989/12/30

Y1 - 1989/12/30

N2 - Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.

AB - Expression of three types of mRNA encoding amyloid β-protein precursor (APP) in various tissues was analysed, using a ribonuclease protection assay, with special reference to Alzheimer's disease (AD). The total content and the proportion of APP mRNAs were specific to each tissue. Among eight tissues examined, the brain was distinct in that the expression level was highest and APP695 mRNA was expressed in abundance. The ratio of APP770/APP751/APP695 mRNAs was approximately 1:10:20 in the cerebral cortex of control brain. The proportions of APP770 mRNA and APP770-plus-APP751 mRNAs increased up to 2.6- and 1.4-fold, respectively, in various regions of AD brain compared with control. The enhanced expression of protease inhibitor-haboring types (APP770 and APP751) may disturb the balance between biosynthesis and degradation of APPs and ultimately lead to accumulation of β-protein as amyloid.

UR - http://www.scopus.com/inward/record.url?scp=0024847273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024847273&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(89)92760-5

DO - 10.1016/0006-291X(89)92760-5

M3 - Article

C2 - 2514687

AN - SCOPUS:0024847273

VL - 165

SP - 1406

EP - 1414

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -