TNF-α and IL-6 signals from the bone marrow derived cells are necessary for normal murine liver regeneration

Kaori Sudo, Yasuhiro Yamada, Kuniaki Saito, Syogo Shimizu, Hazuki Ohashi, Tomohiro Kato, Hisataka Moriwaki, Hiroyasu Ito, Mitsuru Seishima

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29 Citations (Scopus)


In the present study, we used bone marrow transplanted mice and revealed the role of bone marrow derived cells in liver regeneration after partial hepatectomy (PH). Irradiated wild type (WT) mice received a bone marrow transplant from either WT, TNF (tumor necrosis factor)-α knockout (KO), or interleukin (IL)-6 KO donors. Both TNF-α KO- and IL-6 KO-transplanted mice compared with WT-transplanted mice showed decreased hepatocyte DNA synthesis after PH. TNF-α KO-transplanted mice showed no nuclear factor kappa B (NF-κB) and signal transducer and activator of transcription (STAT) 3 binding after PH, while IL-6 KO-transplanted mice showed NF-κB, but not STAT3, binding. Lack of AP-1 or C/EBP binding or expression of c-jun or c-myc mRNA after PH was unrelated to the timing and amount of DNA replication. In conclusion, The TNF-α and IL-6 signals from the blood are necessary for liver regeneration and NF-κB and STAT3 binding are activated via TNF-α and IL-6 signal pathways.

Original languageEnglish
Pages (from-to)671-679
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Issue number11
Publication statusPublished - 01-11-2008


All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology

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