Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan

Satoshi Shimada, Kotaro Aoki, Takeshi Nabeshima, Yu Fuxun, Yohei Kurosaki, Kazuya Shiogama, Takanori Onouchi, Miako Sakaguchi, Takeshi Fuchigami, Hokuto Ono, Kodai Nishi, Guillermo Posadas-Herrera, Leo Uchida, Yuki Takamatsu, Jiro Yasuda, Yutaka Tsutsumi, Hiromi Fujita, Kouichi Morita, Daisuke Hayasaka

Research output: Contribution to journalArticle

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Abstract

Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of 18 F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.

Original languageEnglish
Article number20213
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 11-02-2016

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Nairovirus
Crimean Hemorrhagic Fever
Congo
Ticks
Japan
Viruses
Bunyaviridae
Cercopithecus aethiops
Neutralization Tests
Vero Cells
HEK293 Cells
Fluorodeoxyglucose F18
Infection
Cultured Cells
Genome

All Science Journal Classification (ASJC) codes

  • General

Cite this

Shimada, Satoshi ; Aoki, Kotaro ; Nabeshima, Takeshi ; Fuxun, Yu ; Kurosaki, Yohei ; Shiogama, Kazuya ; Onouchi, Takanori ; Sakaguchi, Miako ; Fuchigami, Takeshi ; Ono, Hokuto ; Nishi, Kodai ; Posadas-Herrera, Guillermo ; Uchida, Leo ; Takamatsu, Yuki ; Yasuda, Jiro ; Tsutsumi, Yutaka ; Fujita, Hiromi ; Morita, Kouichi ; Hayasaka, Daisuke. / Tofla virus : A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. In: Scientific reports. 2016 ; Vol. 6.
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abstract = "Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of 18 F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.",
author = "Satoshi Shimada and Kotaro Aoki and Takeshi Nabeshima and Yu Fuxun and Yohei Kurosaki and Kazuya Shiogama and Takanori Onouchi and Miako Sakaguchi and Takeshi Fuchigami and Hokuto Ono and Kodai Nishi and Guillermo Posadas-Herrera and Leo Uchida and Yuki Takamatsu and Jiro Yasuda and Yutaka Tsutsumi and Hiromi Fujita and Kouichi Morita and Daisuke Hayasaka",
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Shimada, S, Aoki, K, Nabeshima, T, Fuxun, Y, Kurosaki, Y, Shiogama, K, Onouchi, T, Sakaguchi, M, Fuchigami, T, Ono, H, Nishi, K, Posadas-Herrera, G, Uchida, L, Takamatsu, Y, Yasuda, J, Tsutsumi, Y, Fujita, H, Morita, K & Hayasaka, D 2016, 'Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan', Scientific reports, vol. 6, 20213. https://doi.org/10.1038/srep20213

Tofla virus : A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan. / Shimada, Satoshi; Aoki, Kotaro; Nabeshima, Takeshi; Fuxun, Yu; Kurosaki, Yohei; Shiogama, Kazuya; Onouchi, Takanori; Sakaguchi, Miako; Fuchigami, Takeshi; Ono, Hokuto; Nishi, Kodai; Posadas-Herrera, Guillermo; Uchida, Leo; Takamatsu, Yuki; Yasuda, Jiro; Tsutsumi, Yutaka; Fujita, Hiromi; Morita, Kouichi; Hayasaka, Daisuke.

In: Scientific reports, Vol. 6, 20213, 11.02.2016.

Research output: Contribution to journalArticle

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T2 - A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan

AU - Shimada, Satoshi

AU - Aoki, Kotaro

AU - Nabeshima, Takeshi

AU - Fuxun, Yu

AU - Kurosaki, Yohei

AU - Shiogama, Kazuya

AU - Onouchi, Takanori

AU - Sakaguchi, Miako

AU - Fuchigami, Takeshi

AU - Ono, Hokuto

AU - Nishi, Kodai

AU - Posadas-Herrera, Guillermo

AU - Uchida, Leo

AU - Takamatsu, Yuki

AU - Yasuda, Jiro

AU - Tsutsumi, Yutaka

AU - Fujita, Hiromi

AU - Morita, Kouichi

AU - Hayasaka, Daisuke

PY - 2016/2/11

Y1 - 2016/2/11

N2 - Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of 18 F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.

AB - Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of 18 F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.

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