Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model

Hiroki Kakita, Mohamed H. Hussein, Ghada A. Daoud, Takenori Kato, Hiroki Murai, Takahiro Sugiura, Keisuke Mizuno, Yasumasa Yamada, Tetsuya Ito, Sumio Fukuda, Ineko Kato, Satoshi Suzuki, Hajime Togari

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n = 7) and sham (n = 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) α, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-α at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.

Original languageEnglish
Pages (from-to)675-679
Number of pages5
JournalPediatric Research
Volume60
Issue number6
DOIs
Publication statusPublished - 01-12-2006
Externally publishedYes

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Hydrogen Peroxide
Ligation
Antioxidants
Septic Shock
Oxidants
Interleukin-6
Tumor Necrosis Factor-alpha
Neonatal Sepsis
Serum
Interleukin-10
Sepsis

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Kakita, H., Hussein, M. H., Daoud, G. A., Kato, T., Murai, H., Sugiura, T., ... Togari, H. (2006). Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model. Pediatric Research, 60(6), 675-679. https://doi.org/10.1203/01.pdr.0000245911.79943.8a
Kakita, Hiroki ; Hussein, Mohamed H. ; Daoud, Ghada A. ; Kato, Takenori ; Murai, Hiroki ; Sugiura, Takahiro ; Mizuno, Keisuke ; Yamada, Yasumasa ; Ito, Tetsuya ; Fukuda, Sumio ; Kato, Ineko ; Suzuki, Satoshi ; Togari, Hajime. / Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model. In: Pediatric Research. 2006 ; Vol. 60, No. 6. pp. 675-679.
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Kakita, H, Hussein, MH, Daoud, GA, Kato, T, Murai, H, Sugiura, T, Mizuno, K, Yamada, Y, Ito, T, Fukuda, S, Kato, I, Suzuki, S & Togari, H 2006, 'Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model', Pediatric Research, vol. 60, no. 6, pp. 675-679. https://doi.org/10.1203/01.pdr.0000245911.79943.8a

Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model. / Kakita, Hiroki; Hussein, Mohamed H.; Daoud, Ghada A.; Kato, Takenori; Murai, Hiroki; Sugiura, Takahiro; Mizuno, Keisuke; Yamada, Yasumasa; Ito, Tetsuya; Fukuda, Sumio; Kato, Ineko; Suzuki, Satoshi; Togari, Hajime.

In: Pediatric Research, Vol. 60, No. 6, 01.12.2006, p. 675-679.

Research output: Contribution to journalArticle

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T1 - Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model

AU - Kakita, Hiroki

AU - Hussein, Mohamed H.

AU - Daoud, Ghada A.

AU - Kato, Takenori

AU - Murai, Hiroki

AU - Sugiura, Takahiro

AU - Mizuno, Keisuke

AU - Yamada, Yasumasa

AU - Ito, Tetsuya

AU - Fukuda, Sumio

AU - Kato, Ineko

AU - Suzuki, Satoshi

AU - Togari, Hajime

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n = 7) and sham (n = 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) α, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-α at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.

AB - Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n = 7) and sham (n = 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) α, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-α at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.

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