Toward the treatment for Alzheimer’s disease: adsorption is primary mechanism of removing amyloid β protein with hollow-fiber dialyzers of the suitable materials, polysulfone and polymethyl methacrylate

Kazunori Kawaguchi, Akira Saigusa, Shinji Yamada, Takehiro Gotoh, Shigeru Nakai, Yoshiyuki Hiki, Midori Hasegawa, Yukio Yuzawa, Nobuya Kitaguchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The accumulation of amyloid β protein (Aβ) in the brain reflects cognitive impairment in Alzheimer’s disease. We hypothesized that the rapid removal of Aβ from the blood by an extracorporeal system may act as a peripheral Aβ sink from the brain. The present study aimed to determine the optimal materials and modality for Aβ removal by hemodialyzers. In a batch analysis, hollow-fiber fragments of polysulfone (PSf) and polymethyl methacrylate (PMMA) showed greater removal efficiency of Aβ than did other materials, such as cellulose-triacetates and ethylene–vinyl alcohol copolymer (PSf:PMMA at 30 min, 98.6 ± 2.4 %:97.8 ± 0.4 % for Aβ1–40 and 96.6 ± 0.3 %:99.0 ± 1.0 % for Aβ1–42). In a modality study, the Aβ solution was applied to PSf dialyzers and circulated in the dialysis and Air-filled adsorption-mode (i.e., the outer space of the hollow fibers was filled with air) or phosphate-buffered saline (PBS)-filled adsorption modes. The Aβ1–40 removal efficiency of the pre/post dialyzer in the Air-filled adsorption-mode was the highest (62.4 ± 12.6 %, p = 0.007). In a flow rate study in the Air-filled adsorption-mode, 200 mL/min showed the highest Aβ1–40 reduction rate of pool solution (97.3 ± 0.8 % at 15 min) compared with 20 mL/min (p = 0.00001) and 50 mL/min (p = 0.00382). PMMA dialyzers showed similar high reduction rates. Thus, the optimal modality for Aβ removal was the adsorption-mode with PSf or PMMA hollow fibers at around 50 mL/min flow rate, which seems to be suitable for clinical use.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalJournal of Artificial Organs
Volume19
Issue number2
DOIs
Publication statusPublished - 01-06-2016

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Amyloidogenic Proteins
Polysulfones
Polymethyl Methacrylate
Polymethyl methacrylates
Adsorption
Alzheimer Disease
Air
Fibers
Brain
Hemodialyzers
Flow rate
Artificial Kidneys
Serum Amyloid A Protein
Dialysis
Cellulose
Phosphates
Alcohols
Blood
Copolymers
polysulfone P 1700

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Toward the treatment for Alzheimer’s disease: adsorption is primary mechanism of removing amyloid β protein with hollow-fiber dialyzers of the suitable materials, polysulfone and polymethyl methacrylate",
abstract = "The accumulation of amyloid β protein (Aβ) in the brain reflects cognitive impairment in Alzheimer’s disease. We hypothesized that the rapid removal of Aβ from the blood by an extracorporeal system may act as a peripheral Aβ sink from the brain. The present study aimed to determine the optimal materials and modality for Aβ removal by hemodialyzers. In a batch analysis, hollow-fiber fragments of polysulfone (PSf) and polymethyl methacrylate (PMMA) showed greater removal efficiency of Aβ than did other materials, such as cellulose-triacetates and ethylene–vinyl alcohol copolymer (PSf:PMMA at 30 min, 98.6 ± 2.4 {\%}:97.8 ± 0.4 {\%} for Aβ1–40 and 96.6 ± 0.3 {\%}:99.0 ± 1.0 {\%} for Aβ1–42). In a modality study, the Aβ solution was applied to PSf dialyzers and circulated in the dialysis and Air-filled adsorption-mode (i.e., the outer space of the hollow fibers was filled with air) or phosphate-buffered saline (PBS)-filled adsorption modes. The Aβ1–40 removal efficiency of the pre/post dialyzer in the Air-filled adsorption-mode was the highest (62.4 ± 12.6 {\%}, p = 0.007). In a flow rate study in the Air-filled adsorption-mode, 200 mL/min showed the highest Aβ1–40 reduction rate of pool solution (97.3 ± 0.8 {\%} at 15 min) compared with 20 mL/min (p = 0.00001) and 50 mL/min (p = 0.00382). PMMA dialyzers showed similar high reduction rates. Thus, the optimal modality for Aβ removal was the adsorption-mode with PSf or PMMA hollow fibers at around 50 mL/min flow rate, which seems to be suitable for clinical use.",
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Toward the treatment for Alzheimer’s disease : adsorption is primary mechanism of removing amyloid β protein with hollow-fiber dialyzers of the suitable materials, polysulfone and polymethyl methacrylate. / Kawaguchi, Kazunori; Saigusa, Akira; Yamada, Shinji; Gotoh, Takehiro; Nakai, Shigeru; Hiki, Yoshiyuki; Hasegawa, Midori; Yuzawa, Yukio; Kitaguchi, Nobuya.

In: Journal of Artificial Organs, Vol. 19, No. 2, 01.06.2016, p. 149-158.

Research output: Contribution to journalArticle

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T1 - Toward the treatment for Alzheimer’s disease

T2 - adsorption is primary mechanism of removing amyloid β protein with hollow-fiber dialyzers of the suitable materials, polysulfone and polymethyl methacrylate

AU - Kawaguchi, Kazunori

AU - Saigusa, Akira

AU - Yamada, Shinji

AU - Gotoh, Takehiro

AU - Nakai, Shigeru

AU - Hiki, Yoshiyuki

AU - Hasegawa, Midori

AU - Yuzawa, Yukio

AU - Kitaguchi, Nobuya

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