Toxoid flocculation assay by laser light-scattering

Masaaki Iwaki; Yoshinobu Horiuchi; Takako Komiya; Tadashi Fukuda; Yoshichika Arakawa; Motohide Takahashi

doi:10.1016/j.jim.2006.10.012

Toxoid flocculation assay by laser light-scattering

Masaaki Iwaki, Yoshinobu Horiuchi, Takako Komiya, Tadashi Fukuda, Yoshichika Arakawa, Motohide Takahashi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.

Original language	English
Pages (from-to)	138-146
Number of pages	9
Journal	Journal of immunological methods
Volume	318
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2006.10.012
Publication status	Published - 10-01-2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jim.2006.10.012

Cite this

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title = "Toxoid flocculation assay by laser light-scattering",

abstract = "Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.",

author = "Masaaki Iwaki and Yoshinobu Horiuchi and Takako Komiya and Tadashi Fukuda and Yoshichika Arakawa and Motohide Takahashi",

year = "2007",

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language = "English",

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journal = "Journal of immunological methods",

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T1 - Toxoid flocculation assay by laser light-scattering

AU - Iwaki, Masaaki

AU - Horiuchi, Yoshinobu

AU - Komiya, Takako

AU - Fukuda, Tadashi

AU - Arakawa, Yoshichika

AU - Takahashi, Motohide

PY - 2007/1/10

Y1 - 2007/1/10

N2 - Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.

AB - Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.

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M3 - Article

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AN - SCOPUS:33845882993

SN - 0022-1759

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SP - 138

EP - 146

JO - Journal of immunological methods

JF - Journal of immunological methods

IS - 1-2

ER -

Toxoid flocculation assay by laser light-scattering

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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