Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species

Fumio Takizawa, Kyosuke Araki, Maki Ohtani, Hideaki Toda, Yasutaka Saito, Veronica Soto Lampe, J・m Dijkstra, Mitsuru Ototake, Tadaaki Moritomo, Teruyuki Nakanishi, Uwe Fischer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8+ T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM+ lymphocytes, while relatively abundant in IgM-/CD8α+ and IgM-/CD8α- populations. Moreover, we sorted trout CD8α+ lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α+ lymphocytes and/or multiple modes that drive IFN-γ expressions.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalFish and Shellfish Immunology
Volume36
Issue number1
DOIs
Publication statusPublished - 01-01-2014

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Lymphocytes
Transcription
teleost
lymphocytes
transcription (genetics)
Genes
Tissue
gene
Immunoglobulin M
T-cells
genes
rainbow
Oncorhynchus mykiss
cytokines
Transcription Factors
transcription factors
T-lymphocytes
Carassius
Cytokines
neurogenesis

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Takizawa, F., Araki, K., Ohtani, M., Toda, H., Saito, Y., Lampe, V. S., ... Fischer, U. (2014). Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species. Fish and Shellfish Immunology, 36(1), 215-222. https://doi.org/10.1016/j.fsi.2013.11.004
Takizawa, Fumio ; Araki, Kyosuke ; Ohtani, Maki ; Toda, Hideaki ; Saito, Yasutaka ; Lampe, Veronica Soto ; Dijkstra, J・m ; Ototake, Mitsuru ; Moritomo, Tadaaki ; Nakanishi, Teruyuki ; Fischer, Uwe. / Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species. In: Fish and Shellfish Immunology. 2014 ; Vol. 36, No. 1. pp. 215-222.
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Takizawa, F, Araki, K, Ohtani, M, Toda, H, Saito, Y, Lampe, VS, Dijkstra, J, Ototake, M, Moritomo, T, Nakanishi, T & Fischer, U 2014, 'Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species', Fish and Shellfish Immunology, vol. 36, no. 1, pp. 215-222. https://doi.org/10.1016/j.fsi.2013.11.004

Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species. / Takizawa, Fumio; Araki, Kyosuke; Ohtani, Maki; Toda, Hideaki; Saito, Yasutaka; Lampe, Veronica Soto; Dijkstra, J・m; Ototake, Mitsuru; Moritomo, Tadaaki; Nakanishi, Teruyuki; Fischer, Uwe.

In: Fish and Shellfish Immunology, Vol. 36, No. 1, 01.01.2014, p. 215-222.

Research output: Contribution to journalArticle

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T1 - Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species

AU - Takizawa, Fumio

AU - Araki, Kyosuke

AU - Ohtani, Maki

AU - Toda, Hideaki

AU - Saito, Yasutaka

AU - Lampe, Veronica Soto

AU - Dijkstra, J・m

AU - Ototake, Mitsuru

AU - Moritomo, Tadaaki

AU - Nakanishi, Teruyuki

AU - Fischer, Uwe

PY - 2014/1/1

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N2 - Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8+ T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM+ lymphocytes, while relatively abundant in IgM-/CD8α+ and IgM-/CD8α- populations. Moreover, we sorted trout CD8α+ lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α+ lymphocytes and/or multiple modes that drive IFN-γ expressions.

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