TY - JOUR
T1 - Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species
AU - Takizawa, Fumio
AU - Araki, Kyosuke
AU - Ohtani, Maki
AU - Toda, Hideaki
AU - Saito, Yasutaka
AU - Lampe, Veronica Soto
AU - Dijkstra, Johannes Martinus
AU - Ototake, Mitsuru
AU - Moritomo, Tadaaki
AU - Nakanishi, Teruyuki
AU - Fischer, Uwe
N1 - Funding Information:
We thank Carsten Kühn for providing rainbow trout “Born”, and Mrs. Helga Noack and Mr. Toni Krebs for excellent technical assistance. We also thank Bernd Köllner for providing antibodies against IgM. This study was supported by the European Union’ Sixth Framework program (Integrated Project “IMAQUANIM” CT-2005-Food-007103) and by the Alexander von Humboldt Foundation .
PY - 2014/1
Y1 - 2014/1
N2 - Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8+ T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM+ lymphocytes, while relatively abundant in IgM-/CD8α+ and IgM-/CD8α- populations. Moreover, we sorted trout CD8α+ lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α+ lymphocytes and/or multiple modes that drive IFN-γ expressions.
AB - Eomesodermin (Eomes), a T-box transcription factor, is a key molecule associated with function and differentiation of CD8+ T cells and NK cells. Previously, two teleost Eomes genes (Eomes-a and -b), which are located on different chromosomes, were identified and shown to be expressed in zebrafish lymphocytes. For the present study, we identified these genes in rainbow trout and ginbuna crucian carp. Deduced Eomes-a and -b amino acid sequences in both fish species contain a highly conserved T-box DNA binding domain. In RT-PCR, both Eomes transcripts were readily detectable in a variety of tissues in rainbow trout and ginbuna. The high expression of Eomes-a and -b in brain and ovary suggests involvement in neurogenesis and oogenesis, respectively, while their expression in lymphoid tissues presumably is associated with immune functions. Investigation of separated lymphocyte populations from pronephros indicated that both Eomes-a and -b transcripts were few or absent in IgM+ lymphocytes, while relatively abundant in IgM-/CD8α+ and IgM-/CD8α- populations. Moreover, we sorted trout CD8α+ lymphocytes from mucosal and non-mucosal lymphoid tissues and compared the expression profiles of Eomes-a and -b with those of other T cell-related transcription factor genes (GATA-3, T-bet and Runx3), a Th1 cytokine gene (IFN-γ) and a Th2 cytokine gene (IL-4/13A). Interestingly, the tissue distribution of Eomes-a/b, T-bet, and Runx3 versus IFN-γ transcripts did not reveal simple correlations, suggesting tissue-specific properties of CD8α+ lymphocytes and/or multiple modes that drive IFN-γ expressions.
UR - http://www.scopus.com/inward/record.url?scp=84891143615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891143615&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2013.11.004
DO - 10.1016/j.fsi.2013.11.004
M3 - Article
C2 - 24239596
AN - SCOPUS:84891143615
SN - 1050-4648
VL - 36
SP - 215
EP - 222
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 1
ER -