Transcriptional activation of the cdc2 gene is associated with Fas- induced apoptosis of human hematopoietic cells

Yusuke Furukawa, Satsuki Iwase, Yasuhito Terui, Jiro Kikuchi, Takao Sakai, Mitsuru Nakamura, Seiichi Kitagawa, Masatoshi Kitagawa

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63 Citations (Scopus)


Apoptosis has recently been hypothesized to be the result of aberrant cell cycle control. In this study, we have investigated the role of cell cycle-regulatory elements in Fas-induced apoptosis of hematopoietic cells. When HL-60 cells were treated with anti-Fas antibody, rapid activation of growth-associated histone H1 kinase was observed without any change in cell cycle distribution. This was accompanied by the increase in cdc2 mRNA expression and Cdc2 kinase activity. Up-regulation of cdc2 mRNA was similarly induced in BCL-2-overexpressing HL-60 subline by anti-Fas treatment independently of the appearance of apoptotic phenotypes. Fas-induced apoptosis was completely inhibited by butyrolactone I, a specific inhibitor of Cdc2 kinase. Moreover, the same phenomenon was observed during Fas- induced but not spontaneous apoptosis of postmitotic granulocytes. Finally, we have found that 'Fas-responsive element' was located between nucleotides - 730 and -552 of the cdc2 promoter and was responsive for transcriptional activation of the cdc2 gene during Fas-induced apoptosis. These results indicate that aberrant activation of Cdc2 is associated with Fas-induced apoptosis of hematopoietic cells, and that the mechanism of cdc2 transcription during Fas-induced apoptosis is different from that in normal cell cycle control.

Original languageEnglish
Pages (from-to)28469-28477
Number of pages9
JournalJournal of Biological Chemistry
Issue number45
Publication statusPublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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