Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines

Hiroya Yamada, Toru Takano, Fumio Matsuzuka, Mikio Watanabe, Akira Miyauchi, Yoshinori Iwatani

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2 Citations (Scopus)


Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription start site of exon 1 of the TTF-1 gene. However, we did not observe any difference in promoter activity in the region up to -2.6 kb from the proximal transcription start site of the TTF-1 gene between TTF-1 positive and negative cells. These results suggest that the proximal 5'-flanking region of the human TTF -1 gene does not contain sufficient cis-active regulatory information to direct gene expression in thyroid cells,and that other cis- or trans-acting factors participate in the thyroid specific gene expression of TTF-1.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalGenetics and Molecular Biology
Issue number1
Publication statusPublished - 15-02-2011
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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