TY - JOUR
T1 - Transcriptomic profiling predicts multiple pathways and molecules associated with the metastatic phenotype of oral cancer cells
AU - Ideta, Yuka
AU - Tagawa, Takanobu
AU - Hayashi, Yuichiro
AU - Baba, Junichi
AU - Takahashi, Kimiko
AU - Mitsudo, Kenji
AU - Sakurai, Kouhei
N1 - Funding Information:
The Authors are grateful to Advanced Medical Research Center, Yokohama City University for technical support of IPA. The authors thank members of the Department of Nutrition and Dietetics, Kamaukura Women’s University for helpful cooperation and discussion. And the authors also thank Enago (https://www.enago.jp/) for the English language review. This research was funded by Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (grant number 18K09545 and 20K18705).
Funding Information:
The Authors are grateful to Advanced Medical Research Center, Yokohama City University for technical support of IPA. The authors thank members of the Department of Nutrition and Dietetics, Kamaukura Women's University for helpful cooperation and discussion. And the authors also thank Enago (https://www.enago.jp/) for the English language review. This research was funded by Grantsin-Aid for Scientific Research from Japan Society for the Promotion of Science (grant number 18K09545 and 20K18705).
Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Background/Aim: Metastasis to cervical lymph nodes of oral squamous cell carcinoma (OSCC) leads to a poor prognosis. The present study aimed at investigating the pathways and molecules associated with OSCC metastasis. Materials and Methods: The transcriptome between HSC-3 cells and their highly metastatic subline, HSC-3-M3 cells, was examined using gene expression microarray. Gene enrichment analyses and Ingenuity Pathway Analysis were performed. Kaplan-Meier plot analysis using a publicly available dataset was conducted to assess whether candidate molecules are prognosticators. Results: A total of 1,018 genes were differentially expressed, and the inflammatory pathway and NF-κB were predicted to be activated in HSC-3-M3 cells. CSF2 was suggested to be an indicator of poor prognosis in head and neck cancers. Conclusion: Inflammation and NF-κB may be involved in the metastasis of OSCC, and CSF2 is a promising diagnostic and therapeutic molecule. Moreover, HSC-3-M3 cells are a useful cell line model for studying OSCC progression.
AB - Background/Aim: Metastasis to cervical lymph nodes of oral squamous cell carcinoma (OSCC) leads to a poor prognosis. The present study aimed at investigating the pathways and molecules associated with OSCC metastasis. Materials and Methods: The transcriptome between HSC-3 cells and their highly metastatic subline, HSC-3-M3 cells, was examined using gene expression microarray. Gene enrichment analyses and Ingenuity Pathway Analysis were performed. Kaplan-Meier plot analysis using a publicly available dataset was conducted to assess whether candidate molecules are prognosticators. Results: A total of 1,018 genes were differentially expressed, and the inflammatory pathway and NF-κB were predicted to be activated in HSC-3-M3 cells. CSF2 was suggested to be an indicator of poor prognosis in head and neck cancers. Conclusion: Inflammation and NF-κB may be involved in the metastasis of OSCC, and CSF2 is a promising diagnostic and therapeutic molecule. Moreover, HSC-3-M3 cells are a useful cell line model for studying OSCC progression.
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U2 - 10.21873/CGP.20238
DO - 10.21873/CGP.20238
M3 - Article
C2 - 33419893
AN - SCOPUS:85099721261
VL - 18
SP - 17
EP - 27
JO - Cancer Genomics and Proteomics
JF - Cancer Genomics and Proteomics
SN - 1109-6535
IS - 1
ER -