Transdifferentiation of human adipose tissue-derived stromal cells into insulin-producing clusters

Hanayuki Okura, Hiroshi Komoda, Yuichi Fumimoto, Chun Man Lee, Toshirou Nishida, Yoshiki Sawa, Akifumi Matsuyama

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Type 1 diabetes mellitus is caused by autoimmune destruction of insulin-producing beta cells. The major obstacle to transplantation of insulin-producing cells to cure the disease is the limited source of these cells. To overcome this problem, we describe here a multistep protocol for generation of insulin-producing islet-like clusters from human adipose tissue-derived stromal cells (ADSCs). Analysis using reverse transcription polymerase chain reaction detected enhanced expression of various pancreatic genes during the differentiation of ADSCs. Immunofluorescence analysis revealed functional similarities between cells derived from ADSCs and pancreatic islet cells, i.e., the presence of insulin- and C-peptide-coexpressing cells in the clusters and glucagon expression on the cell surface. The glucose challenge tests revealed the production of insulin, and such production was regulated via physiological signaling pathways. Our insulin-producing cells derived from ADSCs could be potentially used for cell therapy of type 1 diabetes mellitus.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalJournal of Artificial Organs
Volume12
Issue number2
DOIs
Publication statusPublished - 01-06-2009
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine
  • Medicine (miscellaneous)

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