Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

Aya Yoshimura, Yoshitaka Tamai, Takahiro Ochiya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Extracellular vesicles (EVs) are mediators of intercellular communication in diverse cellular functions. Visualizing EVs in vivo is important to elucidate the biogenesis of EVs, and various approaches have been developed for in vivo EV tracking. The ubiquitously expressed tetraspanin CD63 is liberally incorporated into EVs. Thus, fluorescently tagged CD63 has been used in many studies to label EVs. In the present study, we presented isolation and transfer assays for EVs from two transgenic rats expressing CD63-GFP in their body fluids or brains.

Original languageEnglish
Title of host publicationMethods in Enzymology
EditorsSheila Spada, Lorenzo Galluzzi
PublisherAcademic Press Inc.
Pages231-242
Number of pages12
ISBN (Print)9780128206621
DOIs
Publication statusPublished - 01-2020

Publication series

NameMethods in Enzymology
Volume645
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles'. Together they form a unique fingerprint.

Cite this