Abstract
It is essential to develop animal models to study the role of genetic factors in the relapse of drug-seeking behavior in genetically engineered mutant mice. This paper reports a typical model of drug-primed and cue-induced reinstatement of extinguished methamphetamine (METH)-seeking behavior in mice. C57BL/6J mice were trained to self-administer METH (0.1 mg/kg/infusion) by poking their nose into an active hole under a fixed ratio schedule in daily 3-h sessions. After acquiring stable METH self-administration behavior, the mice were subjected to extinction training in the absence of both METH and METH-associated cues. Once the active nose-poking responses were extinguished, drug-primed and cue-induced reinstatement were investigated according to a within-subjects design. A priming injection of METH reliably reinstated the extinguished drug-seeking behavior in the absence of both METH and METH-associated cues. Interestingly, the drug-primed METH-seeking behavior disappeared within 2 months after withdrawal from METH, while cue-induced reinstatement of extinguished METH-seeking behavior lasted for at least 5 months after the withdrawal. A correlation study revealed that drug-primed, but not cue-induced, reinstatement behavior was positively correlated with the total amount of METH taken by individuals during METH self-administration. In conclusion, our findings suggest that the present reinstatement procedure for mouse model of relapse is useful and reliable, and different neural mechanisms may be involved in drug-primed and cue-induced METH-seeking behavior.
| Original language | English |
|---|---|
| Pages (from-to) | 261-268 |
| Number of pages | 8 |
| Journal | Behavioural Brain Research |
| Volume | 177 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 27-02-2007 |
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All Science Journal Classification (ASJC) codes
- Behavioral Neuroscience
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Transient drug-primed but persistent cue-induced reinstatement of extinguished methamphetamine-seeking behavior in mice. / Yan, Yijin; Yamada, Kiyofumi; Nitta, Atsumi; Nabeshima, Toshitaka.
In: Behavioural Brain Research, Vol. 177, No. 2, 27.02.2007, p. 261-268.Research output: Contribution to journal › Article
TY - JOUR
T1 - Transient drug-primed but persistent cue-induced reinstatement of extinguished methamphetamine-seeking behavior in mice
AU - Yan, Yijin
AU - Yamada, Kiyofumi
AU - Nitta, Atsumi
AU - Nabeshima, Toshitaka
PY - 2007/2/27
Y1 - 2007/2/27
N2 - It is essential to develop animal models to study the role of genetic factors in the relapse of drug-seeking behavior in genetically engineered mutant mice. This paper reports a typical model of drug-primed and cue-induced reinstatement of extinguished methamphetamine (METH)-seeking behavior in mice. C57BL/6J mice were trained to self-administer METH (0.1 mg/kg/infusion) by poking their nose into an active hole under a fixed ratio schedule in daily 3-h sessions. After acquiring stable METH self-administration behavior, the mice were subjected to extinction training in the absence of both METH and METH-associated cues. Once the active nose-poking responses were extinguished, drug-primed and cue-induced reinstatement were investigated according to a within-subjects design. A priming injection of METH reliably reinstated the extinguished drug-seeking behavior in the absence of both METH and METH-associated cues. Interestingly, the drug-primed METH-seeking behavior disappeared within 2 months after withdrawal from METH, while cue-induced reinstatement of extinguished METH-seeking behavior lasted for at least 5 months after the withdrawal. A correlation study revealed that drug-primed, but not cue-induced, reinstatement behavior was positively correlated with the total amount of METH taken by individuals during METH self-administration. In conclusion, our findings suggest that the present reinstatement procedure for mouse model of relapse is useful and reliable, and different neural mechanisms may be involved in drug-primed and cue-induced METH-seeking behavior.
AB - It is essential to develop animal models to study the role of genetic factors in the relapse of drug-seeking behavior in genetically engineered mutant mice. This paper reports a typical model of drug-primed and cue-induced reinstatement of extinguished methamphetamine (METH)-seeking behavior in mice. C57BL/6J mice were trained to self-administer METH (0.1 mg/kg/infusion) by poking their nose into an active hole under a fixed ratio schedule in daily 3-h sessions. After acquiring stable METH self-administration behavior, the mice were subjected to extinction training in the absence of both METH and METH-associated cues. Once the active nose-poking responses were extinguished, drug-primed and cue-induced reinstatement were investigated according to a within-subjects design. A priming injection of METH reliably reinstated the extinguished drug-seeking behavior in the absence of both METH and METH-associated cues. Interestingly, the drug-primed METH-seeking behavior disappeared within 2 months after withdrawal from METH, while cue-induced reinstatement of extinguished METH-seeking behavior lasted for at least 5 months after the withdrawal. A correlation study revealed that drug-primed, but not cue-induced, reinstatement behavior was positively correlated with the total amount of METH taken by individuals during METH self-administration. In conclusion, our findings suggest that the present reinstatement procedure for mouse model of relapse is useful and reliable, and different neural mechanisms may be involved in drug-primed and cue-induced METH-seeking behavior.
UR - http://www.scopus.com/inward/record.url?scp=33846427708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846427708&partnerID=8YFLogxK
U2 - 10.1016/j.bbr.2006.11.033
DO - 10.1016/j.bbr.2006.11.033
M3 - Article
C2 - 17182116
AN - SCOPUS:33846427708
VL - 177
SP - 261
EP - 268
JO - Behavioural Brain Research
JF - Behavioural Brain Research
SN - 0166-4328
IS - 2
ER -