Transient expression of M-CSF is important for osteoclast-like cell differentiation in a monocytic leukemia cell line

Hiromoto Aoki, Hidehiko Akiyama, Hiromi Hosoya, Masahiko Souda, Toshie Morioku, Tohru Marunouchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Cells of U937, a human monocytic leukemia cell line, differentiate into macrophages by treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA), whereas cells treated with 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3] continue to grow without undergoing differentiation. When U937 cells were successively treated with TPA and 1,25-(OH)2D3, tartrate-resistant acid phosphatase-positive multinucleated cells appeared at 5 days after the treatment. These osteoclast-like cells released a soluble form of 45Ca from 45Ca-labeled bone particles. These cells were not formed when the order of treatment with TPA and 1,25-(OH)2D3 was reversed. Use of either dexamethasone or interferon-γ (IFN-γ) was effective in inhibiting the formation of these osteoclast-like cells. The expression of c-src, c-fms, and macrophage colony stimulating factor (M-CSF) was induced by TPA treatment; however, TPA induced M-CSF gene transcription was attenuated by the subsequent addition of 1,25-(OH)2D3. Furthermore, both dexamethasone and IFN-γ impaired the attenuation of M-CSF expression, suggesting that the transient expression of M-CSF may be important for the formation of osteoclast-like cells.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalJournal of Cellular Biochemistry
Volume64
Issue number1
DOIs
Publication statusPublished - 01-01-1997

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Transient expression of M-CSF is important for osteoclast-like cell differentiation in a monocytic leukemia cell line'. Together they form a unique fingerprint.

Cite this