Translocation of Ki-ras p21 between membrane and cytoplasm by smg GDS

Motohiro Kawamura, Kozo Kaibuchi, Kiyohiko Kishi, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We have previously shown that smg GDS forms a complex with Ki-ras 4B p21 and thereby stimulates its GDP/GTP exchange reaction. We have shown here that smg GDS inhibits the binding of Ki-ras 4B p21 to membranes and induces the dissociation of prebound Ki-ras 4B p21 from membranes. Moreover, we have found that cotransfection of the smg GDS and Ki-ras 4B p21 cDNAs into COS7 cells increases the ratio of the cytosolic form of ras p21 to the membrane- bound form and the ratio of the GTP-bound form of ras p21 to the GDP-bound form. These results indicate that smg GDS regulates not only the GDP/GTP exchange reaction of Ki-ras 4B p21 but also its translocation between membrane and cytoplasm.

Original languageEnglish
Pages (from-to)832-841
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number3
DOIs
Publication statusPublished - 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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