TY - JOUR
T1 - Transplantation of dendritic cells promotes functional recovery from spinal cord injury in common marmoset
AU - Yaguchi, Masae
AU - Tabuse, Masanao
AU - Ohta, Shigeki
AU - Ohkusu-Tsukada, Kozo
AU - Takeuchi, Tamaki
AU - Yamane, Junichi
AU - Katoh, Hiroyuki
AU - Nakamura, Masaya
AU - Matsuzaki, Yumi
AU - Yamada, Masayuki
AU - Itoh, Toshio
AU - Nomura, Tatsuji
AU - Toyama, Yoshiaki
AU - Okano, Hideyuki
AU - Toda, Masahiro
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and a Grant-in-aid for the Global COE program to Keio University from MEXT. We thank Dr. F. Toyota, Dr. H. Ishi, and Y. Tanioka (Central Institute for Experimental Animals) for their technical assistant with the operations and the animal care of CMs. We thank M. Kokubo, T. Muraki, and S. Teramoto (Keio University School of Medicine) for technical assistance.
PY - 2009/12
Y1 - 2009/12
N2 - We previously reported that implantation of dendritic cells (DCs) into the injured site activates neural stem/progenitor cells (NSPCs) and promotes functional recovery after spinal cord injury (SCI) in mice. Working toward clinical application of DC therapy for SCI, we analyzed whether DCs promote functional recovery after SCI in a non-human primate, the common marmoset (CM). CMs are usually born as dizygotic twins. They are thus natural bone marrow and peripheral blood chimeras due to sharing of the placental circulation between dizygotic twins, leading to functional immune tolerance. In this study, to identify adequate CM donor-and-host pairs, mixed leukocyte reaction (MLR) assays were performed. Then, CM-DCs were generated from the bone marrow of the twin selected to be donor and transplanted into the injured site of the spinal cord of the other twin selected to be host, 7 days after injury. Histological analyses revealed fewer areas of demyelination around the injured site in DC-treated CMs than in controls. Immunohistochemical analysis showed that more motor neurons and corticospinal tracts were preserved after SCI in DC-treated CMs. Motor functions were evaluated using three different behavior tests and earlier functional recovery was observed in DC-treated CMs. These results suggest DC therapy to possibly be beneficial in primates with SCI and that this treatment has potential for clinical application.
AB - We previously reported that implantation of dendritic cells (DCs) into the injured site activates neural stem/progenitor cells (NSPCs) and promotes functional recovery after spinal cord injury (SCI) in mice. Working toward clinical application of DC therapy for SCI, we analyzed whether DCs promote functional recovery after SCI in a non-human primate, the common marmoset (CM). CMs are usually born as dizygotic twins. They are thus natural bone marrow and peripheral blood chimeras due to sharing of the placental circulation between dizygotic twins, leading to functional immune tolerance. In this study, to identify adequate CM donor-and-host pairs, mixed leukocyte reaction (MLR) assays were performed. Then, CM-DCs were generated from the bone marrow of the twin selected to be donor and transplanted into the injured site of the spinal cord of the other twin selected to be host, 7 days after injury. Histological analyses revealed fewer areas of demyelination around the injured site in DC-treated CMs than in controls. Immunohistochemical analysis showed that more motor neurons and corticospinal tracts were preserved after SCI in DC-treated CMs. Motor functions were evaluated using three different behavior tests and earlier functional recovery was observed in DC-treated CMs. These results suggest DC therapy to possibly be beneficial in primates with SCI and that this treatment has potential for clinical application.
UR - http://www.scopus.com/inward/record.url?scp=70350584823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350584823&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2009.08.016
DO - 10.1016/j.neures.2009.08.016
M3 - Article
C2 - 19737583
AN - SCOPUS:70350584823
SN - 0168-0102
VL - 65
SP - 384
EP - 392
JO - Neuroscience Research
JF - Neuroscience Research
IS - 4
ER -