Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets

Junichi Yamane, Masaya Nakamura, Akio Iwanami, Masanori Sakaguchi, Hiroyuki Katoh, Masayuki Yamada, Suketaka Momoshima, Sachiyo Miyao, Ken Ishii, Norikazu Tamaoki, Tatsuji Nomura, Hirotaka James Okano, Yonehiro Kanemura, Yoshiaki Toyama, Hideyuki Okano

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI.

Original languageEnglish
Pages (from-to)1394-1405
Number of pages12
JournalJournal of Neuroscience Research
Volume88
Issue number7
DOIs
Publication statusPublished - 15-05-2010

Fingerprint

Callithrix
Neural Stem Cells
Spinal Cord
Stem Cells
Transplantation
Galectin 1
Green Fluorescent Proteins
Spinal Cord Injuries
Stem Cell Transplantation
human LGALS1 protein
Lentivirus
Oligodendroglia
Hand Strength
Therapeutic Uses
Exercise Test
Astrocytes
Primates
Haplorhini
Cell Differentiation

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Yamane, Junichi ; Nakamura, Masaya ; Iwanami, Akio ; Sakaguchi, Masanori ; Katoh, Hiroyuki ; Yamada, Masayuki ; Momoshima, Suketaka ; Miyao, Sachiyo ; Ishii, Ken ; Tamaoki, Norikazu ; Nomura, Tatsuji ; Okano, Hirotaka James ; Kanemura, Yonehiro ; Toyama, Yoshiaki ; Okano, Hideyuki. / Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets. In: Journal of Neuroscience Research. 2010 ; Vol. 88, No. 7. pp. 1394-1405.
@article{ca256c3a86474836beaf89a79df363f3,
title = "Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets",
abstract = "Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI.",
author = "Junichi Yamane and Masaya Nakamura and Akio Iwanami and Masanori Sakaguchi and Hiroyuki Katoh and Masayuki Yamada and Suketaka Momoshima and Sachiyo Miyao and Ken Ishii and Norikazu Tamaoki and Tatsuji Nomura and Okano, {Hirotaka James} and Yonehiro Kanemura and Yoshiaki Toyama and Hideyuki Okano",
year = "2010",
month = "5",
day = "15",
doi = "10.1002/jnr.22322",
language = "English",
volume = "88",
pages = "1394--1405",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "7",

}

Yamane, J, Nakamura, M, Iwanami, A, Sakaguchi, M, Katoh, H, Yamada, M, Momoshima, S, Miyao, S, Ishii, K, Tamaoki, N, Nomura, T, Okano, HJ, Kanemura, Y, Toyama, Y & Okano, H 2010, 'Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets', Journal of Neuroscience Research, vol. 88, no. 7, pp. 1394-1405. https://doi.org/10.1002/jnr.22322

Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets. / Yamane, Junichi; Nakamura, Masaya; Iwanami, Akio; Sakaguchi, Masanori; Katoh, Hiroyuki; Yamada, Masayuki; Momoshima, Suketaka; Miyao, Sachiyo; Ishii, Ken; Tamaoki, Norikazu; Nomura, Tatsuji; Okano, Hirotaka James; Kanemura, Yonehiro; Toyama, Yoshiaki; Okano, Hideyuki.

In: Journal of Neuroscience Research, Vol. 88, No. 7, 15.05.2010, p. 1394-1405.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets

AU - Yamane, Junichi

AU - Nakamura, Masaya

AU - Iwanami, Akio

AU - Sakaguchi, Masanori

AU - Katoh, Hiroyuki

AU - Yamada, Masayuki

AU - Momoshima, Suketaka

AU - Miyao, Sachiyo

AU - Ishii, Ken

AU - Tamaoki, Norikazu

AU - Nomura, Tatsuji

AU - Okano, Hirotaka James

AU - Kanemura, Yonehiro

AU - Toyama, Yoshiaki

AU - Okano, Hideyuki

PY - 2010/5/15

Y1 - 2010/5/15

N2 - Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI.

AB - Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI.

UR - http://www.scopus.com/inward/record.url?scp=77952044022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952044022&partnerID=8YFLogxK

U2 - 10.1002/jnr.22322

DO - 10.1002/jnr.22322

M3 - Article

C2 - 20091712

AN - SCOPUS:77952044022

VL - 88

SP - 1394

EP - 1405

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 7

ER -