TY - JOUR
T1 - Treatment of persistent corneal epithelial defect by autologous serum application
AU - Tsubota, Kazuo
AU - Goto, Eiki
AU - Shimmura, Shigeto
AU - Shimazaki, Jun
N1 - Funding Information:
Supported by the Japanese Ministry of Health and Welfare, Tokyo, Japan, and by the grant of Hightech Research Center at Tokyo Dental College, Ichikawa, Japan. Also supported by the Medical School Faculty and Alumni Grants of the Keio University Medical School, Tokyo, Japan.
PY - 1999/10/1
Y1 - 1999/10/1
N2 - Objective: To evaluate the efficacy of autologous serum application for the treatment of persistent epithelial defect. Design: Prospective, clinical, noncomparative case series. Participants: A total of 16 eyes were studied. Intervention: Autologous serum was prepared from the patients and diluted to 20% by saline. The patients were instructed to use the autologous serum six to ten times a day. The concentration of vitamin A, epidermal growth factor (EGF), and transforming growth factor-β (TGF-β) was measured at 1 week and 1 month stored in the refrigerator and 1 month and 3 months in the freezer. Main Outcome Measures: Time to closure of epithelial defect. Results: Vitamin A, EGF, and TGF-β were stable during the 1 month in the refrigerator and 3 months in the freezer. Among 16 persistent epithelial defects, 7 (43.8%) healed within 2 weeks, 3 (18.8%) healed within 1 month, and the remaining 6 (37.5%) did not respond within 1 month. No apparent side effect of autologous serum application was observed. Conclusions: Autologous serum application healed 43.8% of persistent defect within 2 weeks and 62.5% within 1 month.
AB - Objective: To evaluate the efficacy of autologous serum application for the treatment of persistent epithelial defect. Design: Prospective, clinical, noncomparative case series. Participants: A total of 16 eyes were studied. Intervention: Autologous serum was prepared from the patients and diluted to 20% by saline. The patients were instructed to use the autologous serum six to ten times a day. The concentration of vitamin A, epidermal growth factor (EGF), and transforming growth factor-β (TGF-β) was measured at 1 week and 1 month stored in the refrigerator and 1 month and 3 months in the freezer. Main Outcome Measures: Time to closure of epithelial defect. Results: Vitamin A, EGF, and TGF-β were stable during the 1 month in the refrigerator and 3 months in the freezer. Among 16 persistent epithelial defects, 7 (43.8%) healed within 2 weeks, 3 (18.8%) healed within 1 month, and the remaining 6 (37.5%) did not respond within 1 month. No apparent side effect of autologous serum application was observed. Conclusions: Autologous serum application healed 43.8% of persistent defect within 2 weeks and 62.5% within 1 month.
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U2 - 10.1016/S0161-6420(99)90412-8
DO - 10.1016/S0161-6420(99)90412-8
M3 - Article
C2 - 10519596
AN - SCOPUS:0033208805
SN - 0161-6420
VL - 106
SP - 1984
EP - 1989
JO - Ophthalmology
JF - Ophthalmology
IS - 10
ER -