Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture

Wataru Araki, Nobuya Kitaguchi, Yasuo Tokushima, Kazuhiro Ishii, Hisashi Aratake, Shun Shimohama, Shigenobu Nakamura, Jun Kimura

Research output: Contribution to journalArticlepeer-review

201 Citations (Scopus)


We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 27-11-1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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