TY - JOUR
T1 - Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture
AU - Araki, Wataru
AU - Kitaguchi, Nobuya
AU - Tokushima, Yasuo
AU - Ishii, Kazuhiro
AU - Aratake, Hisashi
AU - Shimohama, Shun
AU - Nakamura, Shigenobu
AU - Kimura, Jun
PY - 1991/11/27
Y1 - 1991/11/27
N2 - We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.
AB - We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.
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U2 - 10.1016/S0006-291X(05)81412-3
DO - 10.1016/S0006-291X(05)81412-3
M3 - Article
C2 - 1958195
AN - SCOPUS:0026318496
SN - 0006-291X
VL - 181
SP - 265
EP - 271
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -