Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture

Wataru Araki, Nobuya Kitaguchi, Yasuo Tokushima, Kazuhiro Ishii, Hisashi Aratake, Shun Shimohama, Shigenobu Nakamura, Jun Kimura

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number1
DOIs
Publication statusPublished - 27-11-1991

Fingerprint

Amyloid beta-Protein Precursor
Neurons
COS Cells
Serum-Free Culture Media
Neurites
Protease Inhibitors
Rats
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Araki, Wataru ; Kitaguchi, Nobuya ; Tokushima, Yasuo ; Ishii, Kazuhiro ; Aratake, Hisashi ; Shimohama, Shun ; Nakamura, Shigenobu ; Kimura, Jun. / Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 1. pp. 265-271.
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Trophic effect of β-amyloid precursor protein on cerebral cortical neurons in culture. / Araki, Wataru; Kitaguchi, Nobuya; Tokushima, Yasuo; Ishii, Kazuhiro; Aratake, Hisashi; Shimohama, Shun; Nakamura, Shigenobu; Kimura, Jun.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 1, 27.11.1991, p. 265-271.

Research output: Contribution to journalArticle

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AU - Araki, Wataru

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