Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells

Keigo Sawada, Masahide Takedachi, Satomi Yamamoto, Chiaki Morimoto, Masao Ozasa, Tomoaki Iwayama, Chun Man Lee, Hanayuki Okura, Akifumi Matsuyama, Masahiro Kitamura, Shinya Murakami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Stem and progenitor cells are currently being investigated for their applicability in cell-based therapy for periodontal tissue regeneration. We recently demonstrated that the transplantation of adipose tissue-derived multi-lineage progenitor cells (ADMPCs) enhances periodontal tissue regeneration in beagle dogs. However, the molecular mechanisms by which transplanted ADMPCs induce periodontal tissue regeneration remain to be elucidated. In this study, trophic factors released by ADMPCs were examined for their paracrine effects on human periodontal ligament cell (HPDL) function. ADMPC conditioned medium (ADMPC-CM) up-regulated osteoblastic gene expression, alkaline phosphatase activity and calcified nodule formation in HPDLs, but did not significantly affect their proliferative response. ADMPCs secreted a number of growth factors, including insulin-like growth factor binding protein 6 (IGFBP6), hepatocyte growth factor and vascular endothelial growth factor. Among these, IGFBP6 was most highly expressed. Interestingly, the positive effects of ADMPC-CM on HPDL differentiation were significantly suppressed by transfecting ADMPCs with IGFBP6 siRNA. Our results suggest that ADMPCs transplanted into a defect in periodontal tissue release trophic factors that can stimulate the differentiation of HPDLs to mineralized tissue-forming cells, such as osteoblasts and cementoblasts. IGFBP6 may play crucial roles in ADMPC-induced periodontal regeneration.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume464
Issue number1
DOIs
Publication statusPublished - 20-07-2015

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Periodontal Ligament
Ligaments
Adipose Tissue
Stem Cells
Tissue
Insulin-Like Growth Factor Binding Protein 6
Tissue regeneration
Regeneration
Dental Cementum
Hepatocyte Growth Factor
Osteoblasts
Conditioned Culture Medium
Cell- and Tissue-Based Therapy
Gene expression
Vascular Endothelial Growth Factor A
Small Interfering RNA
Alkaline Phosphatase
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Transplantation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sawada, Keigo ; Takedachi, Masahide ; Yamamoto, Satomi ; Morimoto, Chiaki ; Ozasa, Masao ; Iwayama, Tomoaki ; Lee, Chun Man ; Okura, Hanayuki ; Matsuyama, Akifumi ; Kitamura, Masahiro ; Murakami, Shinya. / Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 464, No. 1. pp. 299-305.
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Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells. / Sawada, Keigo; Takedachi, Masahide; Yamamoto, Satomi; Morimoto, Chiaki; Ozasa, Masao; Iwayama, Tomoaki; Lee, Chun Man; Okura, Hanayuki; Matsuyama, Akifumi; Kitamura, Masahiro; Murakami, Shinya.

In: Biochemical and Biophysical Research Communications, Vol. 464, No. 1, 20.07.2015, p. 299-305.

Research output: Contribution to journalArticle

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AU - Kitamura, Masahiro

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