TY - JOUR
T1 - Tryptophan metabolism was accelerated by exercise in rat
AU - Ito, Yasuhiro
AU - Yonekura, Reiko
AU - Maruta, Kazuhiro
AU - Koike, Takaaki
AU - Nakagami, Yasushi
AU - Shibata, Katsumi
AU - Saito, Kuniaki
AU - Nagamura, Yoichi
N1 - Funding Information:
The authors gratefully acknowledge a financial support of the Ministry of Education. Science and Culture of Japan (Specially Promoted Research No. 06101004).
PY - 2003
Y1 - 2003
N2 - We have already reported that exercise activates kynurenine pathway. But, the mechanism for this activation by exercise is still unclear. Kynurenine is metabolized to NAD, which is an essential factor for energy metabolism. In this study, exercise on treadmill was loaded to rats until all-out and tryptophan metabolites and tryptophan 2,3-dioxygenase (TDO) and indoleamine 2, 3-dioxygenase (IDO) activities were determined in the blood, livers, and macrophages, respectively, in the exercise-loaded rats. The mean values of serum tryptophan concentration decreased from 92.6±6.0 nmol/ml to 52.4±10.2 nmol/ml (p<0.05) just after treadmill load. The serum kynurenine concentration had increased from 2.06±0.25 nmol/ml to 3.08±0.62 nmol/ml (p<0.005). And whole blood NAD concentration increased from 68.8±14.6 nmol/ml to 77.9±19.1 nmol/ml (p<0.005). These results showed that exercise activated the kynurenine pathway of tryptophan metabolism and made NAD which will be concerned with energy metabolism in mitochondria. Tryptophan-NAD pathway was initiated by cleavage of indole ring of tryptophan by TDO in the liver and IDO in many organs. We had also found that the exercise increase IDO activity of macrophages, but not TDO activity.
AB - We have already reported that exercise activates kynurenine pathway. But, the mechanism for this activation by exercise is still unclear. Kynurenine is metabolized to NAD, which is an essential factor for energy metabolism. In this study, exercise on treadmill was loaded to rats until all-out and tryptophan metabolites and tryptophan 2,3-dioxygenase (TDO) and indoleamine 2, 3-dioxygenase (IDO) activities were determined in the blood, livers, and macrophages, respectively, in the exercise-loaded rats. The mean values of serum tryptophan concentration decreased from 92.6±6.0 nmol/ml to 52.4±10.2 nmol/ml (p<0.05) just after treadmill load. The serum kynurenine concentration had increased from 2.06±0.25 nmol/ml to 3.08±0.62 nmol/ml (p<0.005). And whole blood NAD concentration increased from 68.8±14.6 nmol/ml to 77.9±19.1 nmol/ml (p<0.005). These results showed that exercise activated the kynurenine pathway of tryptophan metabolism and made NAD which will be concerned with energy metabolism in mitochondria. Tryptophan-NAD pathway was initiated by cleavage of indole ring of tryptophan by TDO in the liver and IDO in many organs. We had also found that the exercise increase IDO activity of macrophages, but not TDO activity.
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U2 - 10.1007/978-1-4615-0135-0_61
DO - 10.1007/978-1-4615-0135-0_61
M3 - Article
C2 - 15206771
AN - SCOPUS:1042291123
SN - 0065-2598
VL - 527
SP - 531
EP - 535
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -