TSC-box is essential for the nuclear localization and antiproliferative effect of XTSC-22

Akiko Hashiguchi, Keisuke Hitachi, Masafumi Inui, Koji Okabayashi, Makoto Asashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transforming growth factor-β1-stimulated clone 22 (TSC-22) encodes a leucine zipper-containing protein that is highly conserved among various species. Mammalian TSC-22 is a potential tumor suppressor gene. It translocates into nuclei and suppresses cell division upon antiproliferative stimuli. In human colon carcinoma cells, TSC-22 inhibits cell growth by upregulating expression of the p21 gene, a cyclin-dependent kinase (Cdk) inhibitor. We previously showed that the Xenopus laevis homologue of the TSC-22 gene (XTSC-22) is required for cell movement during gastrulation through cell cycle regulation. In this report, we investigated the molecular mechanism of the antiproliferative effect of XTSC-22. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis suggested that XTSC-22 did not affect the expression levels of the p21 family of Cdk inhibitors or other cell cycle regulators. Analysis of deletion mutants of XTSC-22 revealed that nuclear localization of the N-terminal TSC-box is necessary for cell cycle inhibition by XTSC-22. Further experiments suggested that p27Xic1, a key Cdk inhibitor in Xenopus, interacts with XTSC-22. Because p27Xic1 is a cell cycle inhibitor with a nuclear localization signal, it is possible that XTSC-22 suppresses cell division by translocating into the nucleus with p27Xic1, where it may potentiate the intranuclear action of p27Xic1.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalDevelopment Growth and Differentiation
Volume49
Issue number3
DOIs
Publication statusPublished - 01-04-2007

Fingerprint

Xenopus laevis
Cell Cycle
Genes
Cyclin-Dependent Kinases
Cyclin-Dependent Kinase Inhibitor p21
Cell Nucleus Division
Leucine Zippers
Nuclear Localization Signals
Gastrulation
Transforming Growth Factors
Xenopus
Tumor Suppressor Genes
Reverse Transcriptase Polymerase Chain Reaction
Cell Division
Cell Movement
Colon
Clone Cells
Carcinoma
Gene Expression
Growth

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Hashiguchi, Akiko ; Hitachi, Keisuke ; Inui, Masafumi ; Okabayashi, Koji ; Asashima, Makoto. / TSC-box is essential for the nuclear localization and antiproliferative effect of XTSC-22. In: Development Growth and Differentiation. 2007 ; Vol. 49, No. 3. pp. 197-204.
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TSC-box is essential for the nuclear localization and antiproliferative effect of XTSC-22. / Hashiguchi, Akiko; Hitachi, Keisuke; Inui, Masafumi; Okabayashi, Koji; Asashima, Makoto.

In: Development Growth and Differentiation, Vol. 49, No. 3, 01.04.2007, p. 197-204.

Research output: Contribution to journalArticle

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T1 - TSC-box is essential for the nuclear localization and antiproliferative effect of XTSC-22

AU - Hashiguchi, Akiko

AU - Hitachi, Keisuke

AU - Inui, Masafumi

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AU - Asashima, Makoto

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