Tubulin and CRMP-2 complex is transported via Kinesin-1

Toshihide Kimura, Nariko Arimura, Yuko Fukata, Hiroyasu Watanabe, Akihiro Iwamatsu, Kozo Kaibuchi

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

The transport of tubulin and microtubules in a growing axon is essential for axonal growth and maintenance. However, the molecular mechanism underlying the linkage of tubulin and microtubules to motor proteins is not yet clear. Collapsin response mediator protein-2 (CRMP-2) is enriched at the distal part of growing axons in primary hippocampal neurons and plays a critical role in axon differentiation through its interaction with tubulin dimer and Numb. In this study, we show that CRMP-2 regulates tubulin transport by linking tubulin and Kinesin-1. The C-terminal region of CRMP-2 directly binds to the tetratricopeptide repeat domain of kinesin light chain 1 (KLC1). Soluble tubulin binds to the middle of CRMP-2 and forms a trimeric complex with CRMP-2/KLC1. Furthermore, the movement of GFP-tubulin in the photobleached area is weakened by knockdown of KLCs or CRMP-2. These results indicate that the CRMP-2/Kinesin-1 complex regulates soluble tubulin transport to the distal part of the growing axon.

Original languageEnglish
Pages (from-to)1371-1382
Number of pages12
JournalJournal of neurochemistry
Volume93
Issue number6
DOIs
Publication statusPublished - 06-2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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