Tumor necrosis factor-α from bone marrow-derived cells is not essential for the expression of adhesion molecules in lipopolysaccharide-induced nasal inflammation

Masao Iwasaki, Kuniaki Saito, Kenji Sekikawa, Yasuhiro Yamada, Hisayasu Wada, Keisuke Mizuta, Yatsuji Ito, Mitsuru Seishima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Both the role and source of tumor necrosis factor (TNF-α) in lipopolysaccharide (LPS)-induced nasal inflammation were investigated using TNF-α gene deficient (TNF-α -/-) mice and chimeric mice that are TNF-α gene deficient only in bone marrow-derived cells. In the present study, intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression levels in the nasal mucosa were significantly decreased following intranasal instillation of LPS in TNF-α gene deficient mice compared to those in wild type mice. In contrast, ICAM-1 and VCAM-1 mRNA expressions were not significantly decreased although TNF-α mRNA expression was dramatically decreased in TNF-α gene deficient bone marrow-transplanted-chimeric (TNF-α -/-→+/+) mice compared to those in wild type bone marrow-transplanted-control (TNF-α +/+→+/+) mice. These results indicate that the elevation of TNF-α mRNA in the nasal mucosa is mainly originated from bone marrow-derived cells. However, even low expression of TNF-α at local inflammation sites is sufficient to induce the expression of adhesion molecules in acute LPS-induced experimental rhinitis.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalCytokine
Volume21
Issue number3
DOIs
Publication statusPublished - 07-02-2003
Externally publishedYes

Fingerprint

Nose
Bone Marrow Cells
Lipopolysaccharides
Bone
Adhesion
Tumor Necrosis Factor-alpha
Inflammation
Molecules
Genes
Messenger RNA
Vascular Cell Adhesion Molecule-1
Nasal Mucosa
Bone Marrow
Rhinitis

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Iwasaki, Masao ; Saito, Kuniaki ; Sekikawa, Kenji ; Yamada, Yasuhiro ; Wada, Hisayasu ; Mizuta, Keisuke ; Ito, Yatsuji ; Seishima, Mitsuru. / Tumor necrosis factor-α from bone marrow-derived cells is not essential for the expression of adhesion molecules in lipopolysaccharide-induced nasal inflammation. In: Cytokine. 2003 ; Vol. 21, No. 3. pp. 129-136.
@article{6d81ac3cca964279ad4573a684149f48,
title = "Tumor necrosis factor-α from bone marrow-derived cells is not essential for the expression of adhesion molecules in lipopolysaccharide-induced nasal inflammation",
abstract = "Both the role and source of tumor necrosis factor (TNF-α) in lipopolysaccharide (LPS)-induced nasal inflammation were investigated using TNF-α gene deficient (TNF-α -/-) mice and chimeric mice that are TNF-α gene deficient only in bone marrow-derived cells. In the present study, intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression levels in the nasal mucosa were significantly decreased following intranasal instillation of LPS in TNF-α gene deficient mice compared to those in wild type mice. In contrast, ICAM-1 and VCAM-1 mRNA expressions were not significantly decreased although TNF-α mRNA expression was dramatically decreased in TNF-α gene deficient bone marrow-transplanted-chimeric (TNF-α -/-→+/+) mice compared to those in wild type bone marrow-transplanted-control (TNF-α +/+→+/+) mice. These results indicate that the elevation of TNF-α mRNA in the nasal mucosa is mainly originated from bone marrow-derived cells. However, even low expression of TNF-α at local inflammation sites is sufficient to induce the expression of adhesion molecules in acute LPS-induced experimental rhinitis.",
author = "Masao Iwasaki and Kuniaki Saito and Kenji Sekikawa and Yasuhiro Yamada and Hisayasu Wada and Keisuke Mizuta and Yatsuji Ito and Mitsuru Seishima",
year = "2003",
month = "2",
day = "7",
doi = "10.1016/S1043-4666(03)00030-9",
language = "English",
volume = "21",
pages = "129--136",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "3",

}

Tumor necrosis factor-α from bone marrow-derived cells is not essential for the expression of adhesion molecules in lipopolysaccharide-induced nasal inflammation. / Iwasaki, Masao; Saito, Kuniaki; Sekikawa, Kenji; Yamada, Yasuhiro; Wada, Hisayasu; Mizuta, Keisuke; Ito, Yatsuji; Seishima, Mitsuru.

In: Cytokine, Vol. 21, No. 3, 07.02.2003, p. 129-136.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tumor necrosis factor-α from bone marrow-derived cells is not essential for the expression of adhesion molecules in lipopolysaccharide-induced nasal inflammation

AU - Iwasaki, Masao

AU - Saito, Kuniaki

AU - Sekikawa, Kenji

AU - Yamada, Yasuhiro

AU - Wada, Hisayasu

AU - Mizuta, Keisuke

AU - Ito, Yatsuji

AU - Seishima, Mitsuru

PY - 2003/2/7

Y1 - 2003/2/7

N2 - Both the role and source of tumor necrosis factor (TNF-α) in lipopolysaccharide (LPS)-induced nasal inflammation were investigated using TNF-α gene deficient (TNF-α -/-) mice and chimeric mice that are TNF-α gene deficient only in bone marrow-derived cells. In the present study, intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression levels in the nasal mucosa were significantly decreased following intranasal instillation of LPS in TNF-α gene deficient mice compared to those in wild type mice. In contrast, ICAM-1 and VCAM-1 mRNA expressions were not significantly decreased although TNF-α mRNA expression was dramatically decreased in TNF-α gene deficient bone marrow-transplanted-chimeric (TNF-α -/-→+/+) mice compared to those in wild type bone marrow-transplanted-control (TNF-α +/+→+/+) mice. These results indicate that the elevation of TNF-α mRNA in the nasal mucosa is mainly originated from bone marrow-derived cells. However, even low expression of TNF-α at local inflammation sites is sufficient to induce the expression of adhesion molecules in acute LPS-induced experimental rhinitis.

AB - Both the role and source of tumor necrosis factor (TNF-α) in lipopolysaccharide (LPS)-induced nasal inflammation were investigated using TNF-α gene deficient (TNF-α -/-) mice and chimeric mice that are TNF-α gene deficient only in bone marrow-derived cells. In the present study, intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression levels in the nasal mucosa were significantly decreased following intranasal instillation of LPS in TNF-α gene deficient mice compared to those in wild type mice. In contrast, ICAM-1 and VCAM-1 mRNA expressions were not significantly decreased although TNF-α mRNA expression was dramatically decreased in TNF-α gene deficient bone marrow-transplanted-chimeric (TNF-α -/-→+/+) mice compared to those in wild type bone marrow-transplanted-control (TNF-α +/+→+/+) mice. These results indicate that the elevation of TNF-α mRNA in the nasal mucosa is mainly originated from bone marrow-derived cells. However, even low expression of TNF-α at local inflammation sites is sufficient to induce the expression of adhesion molecules in acute LPS-induced experimental rhinitis.

UR - http://www.scopus.com/inward/record.url?scp=0037423043&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037423043&partnerID=8YFLogxK

U2 - 10.1016/S1043-4666(03)00030-9

DO - 10.1016/S1043-4666(03)00030-9

M3 - Article

VL - 21

SP - 129

EP - 136

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 3

ER -