Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression

Kyoko Kubota, Kiyoshi Inoue, Ryota Hashimoto, Natsuko Kumamoto, Asako Kosuga, Masahiko Tatsumi, Kunitoshi Kamijima, Hiroshi Kunugi, Nakao Iwata, Norio Ozaki, Masatoshi Takeda, Masaya Tohyama

Research output: Contribution to journalArticle

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Abstract

An increase in serum tumor necrosis factor-α (TNF-α) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-α/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-α/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression.

Original languageEnglish
Pages (from-to)496-508
Number of pages13
JournalJournal of Neurochemistry
Volume110
Issue number2
DOIs
Publication statusPublished - 01-07-2009

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Tumor Necrosis Factor Receptors
Cell adhesion
Cadherins
Cell Adhesion
Modulation
Brain
Proteins
Tumor Necrosis Factor-alpha
Neurons
E2F1 Transcription Factor
Phosphorylation
Polymorphism
Dendritic Spines
Small Interfering RNA
Tyrosine
Adhesives
Nucleotides
Genes
Cells
Association reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Kubota, Kyoko ; Inoue, Kiyoshi ; Hashimoto, Ryota ; Kumamoto, Natsuko ; Kosuga, Asako ; Tatsumi, Masahiko ; Kamijima, Kunitoshi ; Kunugi, Hiroshi ; Iwata, Nakao ; Ozaki, Norio ; Takeda, Masatoshi ; Tohyama, Masaya. / Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression. In: Journal of Neurochemistry. 2009 ; Vol. 110, No. 2. pp. 496-508.
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abstract = "An increase in serum tumor necrosis factor-α (TNF-α) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-α/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-α/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression.",
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Kubota, K, Inoue, K, Hashimoto, R, Kumamoto, N, Kosuga, A, Tatsumi, M, Kamijima, K, Kunugi, H, Iwata, N, Ozaki, N, Takeda, M & Tohyama, M 2009, 'Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression', Journal of Neurochemistry, vol. 110, no. 2, pp. 496-508. https://doi.org/10.1111/j.1471-4159.2009.06099.x

Tumor necrosis factor receptor-associated protein 1 regulates cell adhesion and synaptic morphology via modulation of N-cadherin expression. / Kubota, Kyoko; Inoue, Kiyoshi; Hashimoto, Ryota; Kumamoto, Natsuko; Kosuga, Asako; Tatsumi, Masahiko; Kamijima, Kunitoshi; Kunugi, Hiroshi; Iwata, Nakao; Ozaki, Norio; Takeda, Masatoshi; Tohyama, Masaya.

In: Journal of Neurochemistry, Vol. 110, No. 2, 01.07.2009, p. 496-508.

Research output: Contribution to journalArticle

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AU - Kubota, Kyoko

AU - Inoue, Kiyoshi

AU - Hashimoto, Ryota

AU - Kumamoto, Natsuko

AU - Kosuga, Asako

AU - Tatsumi, Masahiko

AU - Kamijima, Kunitoshi

AU - Kunugi, Hiroshi

AU - Iwata, Nakao

AU - Ozaki, Norio

AU - Takeda, Masatoshi

AU - Tohyama, Masaya

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N2 - An increase in serum tumor necrosis factor-α (TNF-α) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-α/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-α/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression.

AB - An increase in serum tumor necrosis factor-α (TNF-α) levels is closely related to the pathogenesis of major depression. However, the underlying molecular mechanism between this increase and impairment of brain function remains elusive. To better understand TNF-α/TNF receptor 1 signaling in the brain, we analyzed the brain distribution and function of tumor necrosis factor receptor-associated protein 1 (TRAP1). Here we show that TRAP1 is broadly expressed in neurons in the mouse brain, including regions that are implicated in the pathogenesis of major depression. We demonstrate that small interfering RNA-mediated knockdown of TRAP1 in a neuronal cell line decreases tyrosine phosphorylation of STAT3, followed by a reduction of the transcription factor E2F1, resulting in a down-regulation of N-cadherin, and affects the adhesive properties of the cells. In addition, in cultured hippocampal neurons, reduced expression of N-cadherin by TRAP1 knockdown influences the morphology of dendritic spines. We also report a significant association between several single nucleotide polymorphisms in the TRAP1 gene and major depression. Our findings indicate that TRAP1 mediates TNF-α/TNF receptor 1 signaling to modulate N-cadherin expression and to regulate cell adhesion and synaptic morphology, which may contribute to the pathogenesis of major depression.

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