Two-dimensional distribution of G(i2α) in the plasma membrane: A critical evaluation by immunocytochemistry

Ryuji Nomura, Chisato Inuo, Yukiko Takahashi, Tomiko Asano, Toyoshi Fujimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalFEBS Letters
Volume415
Issue number2
DOIs
Publication statusPublished - 29-09-1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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