Two-dimensional distribution of G(i2α) in the plasma membrane: A critical evaluation by immunocytochemistry

Ryuji Nomura, Chisato Inuo, Yukiko Takahashi, Tomiko Asano, Toyoshi Fujimoto

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalFEBS Letters
Issue number2
Publication statusPublished - 29-09-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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