Two-dimensional distribution of G(i2α) in the plasma membrane

A critical evaluation by immunocytochemistry

Ryuji Nomura, Chisato Inuo, Yukiko Takahashi, Tomiko Asano, Toyoshi Fujimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalFEBS Letters
Volume415
Issue number2
DOIs
Publication statusPublished - 29-09-1997

Fingerprint

Caveolins
Caveolae
Cell membranes
Immunohistochemistry
Cell Membrane
Signal transduction
Labeling
Ultracentrifugation
Signal Transduction
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Nomura, Ryuji ; Inuo, Chisato ; Takahashi, Yukiko ; Asano, Tomiko ; Fujimoto, Toyoshi. / Two-dimensional distribution of G(i2α) in the plasma membrane : A critical evaluation by immunocytochemistry. In: FEBS Letters. 1997 ; Vol. 415, No. 2. pp. 139-144.
@article{9bef5ddf7d204716baf9dc811ce1946f,
title = "Two-dimensional distribution of G(i2α) in the plasma membrane: A critical evaluation by immunocytochemistry",
abstract = "Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.",
author = "Ryuji Nomura and Chisato Inuo and Yukiko Takahashi and Tomiko Asano and Toyoshi Fujimoto",
year = "1997",
month = "9",
day = "29",
doi = "10.1016/S0014-5793(97)01114-9",
language = "English",
volume = "415",
pages = "139--144",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

Two-dimensional distribution of G(i2α) in the plasma membrane : A critical evaluation by immunocytochemistry. / Nomura, Ryuji; Inuo, Chisato; Takahashi, Yukiko; Asano, Tomiko; Fujimoto, Toyoshi.

In: FEBS Letters, Vol. 415, No. 2, 29.09.1997, p. 139-144.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Two-dimensional distribution of G(i2α) in the plasma membrane

T2 - A critical evaluation by immunocytochemistry

AU - Nomura, Ryuji

AU - Inuo, Chisato

AU - Takahashi, Yukiko

AU - Asano, Tomiko

AU - Fujimoto, Toyoshi

PY - 1997/9/29

Y1 - 1997/9/29

N2 - Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

AB - Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We book G(2α) as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and G(i2α) in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and G(i2α) were found to distribute differently, Secondly, by novel immunocytochemical methods it was found that the labeling density of G(i2α) was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of G(i2α) in caveolae is lower than deduced from most biochemical studies.

UR - http://www.scopus.com/inward/record.url?scp=0031590153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031590153&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(97)01114-9

DO - 10.1016/S0014-5793(97)01114-9

M3 - Article

VL - 415

SP - 139

EP - 144

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -