Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies

Y. Ishii, Akihiro Matsuura, H. Yuasa, H. Narita, T. Takami, K. Kikuchi

Research output: Contribution to journalArticle

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Abstract

Rat thymus and T-cell antigens were defined by using two distinct monoclonal antibodies (R-1-3B3 and R-1-12C5). R-1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and T cells but not B cells and bone marrow cells. The antigen defined by this R-1-3B3 antibody occurred more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R-1-3B3 antibody recognized a single glycoprotein with a molecular weight of 67,000, which were able to interact with Lens culinaris haemagglutinin. R-1-12C5 antibody, on the other hand, reacted with all of thymus and T cells as well as with a subpopulation (approximately 20%) of bone marrow cells. In contrast to the antigen defined by R-1-3B3, that detected by R-1-12C5 antibody existed largely on cortical thymocytes and peripheral T cells. R-1-12C5 antibody detected a single glycoprotein with a 95,000 molecular weight, which could also interact with Lens culinaris haemagglutinin. Based on these data described above and since both antigens defined by R-1-3B3 and R-1-12C5 antibodies were absent from rat brain tissue, we concluded that they were distinct from brain-associated thymic antigens in rats including Thy-1 and W3/13 antigen systems.

Original languageEnglish
Pages (from-to)743-754
Number of pages12
JournalImmunology
Volume48
Issue number4
Publication statusPublished - 02-06-1983

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Thymus Gland
Monoclonal Antibodies
T-Lymphocytes
Antibodies
Antigens
Thymocytes
Lens Plant
Hemagglutinins
Bone Marrow Cells
Glycoproteins
Molecular Weight
Viral Tumor Antigens
Brain
Fluorescent Antibody Technique
B-Lymphocytes
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Ishii, Y., Matsuura, A., Yuasa, H., Narita, H., Takami, T., & Kikuchi, K. (1983). Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. Immunology, 48(4), 743-754.
Ishii, Y. ; Matsuura, Akihiro ; Yuasa, H. ; Narita, H. ; Takami, T. ; Kikuchi, K. / Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. In: Immunology. 1983 ; Vol. 48, No. 4. pp. 743-754.
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Ishii, Y, Matsuura, A, Yuasa, H, Narita, H, Takami, T & Kikuchi, K 1983, 'Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies', Immunology, vol. 48, no. 4, pp. 743-754.

Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. / Ishii, Y.; Matsuura, Akihiro; Yuasa, H.; Narita, H.; Takami, T.; Kikuchi, K.

In: Immunology, Vol. 48, No. 4, 02.06.1983, p. 743-754.

Research output: Contribution to journalArticle

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N2 - Rat thymus and T-cell antigens were defined by using two distinct monoclonal antibodies (R-1-3B3 and R-1-12C5). R-1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and T cells but not B cells and bone marrow cells. The antigen defined by this R-1-3B3 antibody occurred more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R-1-3B3 antibody recognized a single glycoprotein with a molecular weight of 67,000, which were able to interact with Lens culinaris haemagglutinin. R-1-12C5 antibody, on the other hand, reacted with all of thymus and T cells as well as with a subpopulation (approximately 20%) of bone marrow cells. In contrast to the antigen defined by R-1-3B3, that detected by R-1-12C5 antibody existed largely on cortical thymocytes and peripheral T cells. R-1-12C5 antibody detected a single glycoprotein with a 95,000 molecular weight, which could also interact with Lens culinaris haemagglutinin. Based on these data described above and since both antigens defined by R-1-3B3 and R-1-12C5 antibodies were absent from rat brain tissue, we concluded that they were distinct from brain-associated thymic antigens in rats including Thy-1 and W3/13 antigen systems.

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Ishii Y, Matsuura A, Yuasa H, Narita H, Takami T, Kikuchi K. Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. Immunology. 1983 Jun 2;48(4):743-754.