Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies

Y. Ishii, A. Matsuura, H. Yuasa, H. Narita, T. Takami, K. Kikuchi

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Abstract

Rat thymus and T-cell antigens were defined by using two distinct monoclonal antibodies (R-1-3B3 and R-1-12C5). R-1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and T cells but not B cells and bone marrow cells. The antigen defined by this R-1-3B3 antibody occurred more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R-1-3B3 antibody recognized a single glycoprotein with a molecular weight of 67,000, which were able to interact with Lens culinaris haemagglutinin. R-1-12C5 antibody, on the other hand, reacted with all of thymus and T cells as well as with a subpopulation (approximately 20%) of bone marrow cells. In contrast to the antigen defined by R-1-3B3, that detected by R-1-12C5 antibody existed largely on cortical thymocytes and peripheral T cells. R-1-12C5 antibody detected a single glycoprotein with a 95,000 molecular weight, which could also interact with Lens culinaris haemagglutinin. Based on these data described above and since both antigens defined by R-1-3B3 and R-1-12C5 antibodies were absent from rat brain tissue, we concluded that they were distinct from brain-associated thymic antigens in rats including Thy-1 and W3/13 antigen systems.

Original languageEnglish
Pages (from-to)743-754
Number of pages12
JournalImmunology
Volume48
Issue number4
Publication statusPublished - 02-06-1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Ishii, Y., Matsuura, A., Yuasa, H., Narita, H., Takami, T., & Kikuchi, K. (1983). Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. Immunology, 48(4), 743-754.