Two LIM domain proteins and UNC-96 link UNC-97/PINCH to myosin thick filaments in Caenorhabditis elegans muscle

Hiroshi Qadota, Kristina B. Mercer, Rachel K. Miller, Kozo Kaibuchi, Guy M. Benian

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

By yeast two-hybrid screening, we found three novel interactors (UNC-95, LIM-8, and LIM-9) for UNC-97/PINCH in Caenorhabditis elegans. All three proteins contain LIM domains that are required for binding. Among the three interactors, LIM-8 and LIM-9 also bind to UNC-96, a component of sarcomeric M-lines. UNC-96 and LIM-8 also bind to the C-terminal portion of a myosin heavy chain (MHC), MHC A, which resides in the middle of thick filaments in the proximity of M-lines. All interactions identified by yeast two-hybrid assays were confirmed by in vitro binding assays using purified proteins. All three novel UNC-97 interactors are expressed in body wall muscle and by antibodies localize to M-lines. Either a decreased or an increased dosage of UNC-96 results in disorganization of thick filaments. Our previous studies showed that UNC-98, a C2H2 Zn finger protein, acts as a linkage between UNC-97, an integrin-associated protein, and MHC A in myosin thick filaments. In this study, we demonstrate another mechanism by which this linkage occurs: from UNC-97 through LIM-8 or LIM-9/UNC-96 to myosin.

Original languageEnglish
Pages (from-to)4317-4326
Number of pages10
JournalMolecular Biology of the Cell
Volume18
Issue number11
DOIs
Publication statusPublished - 11-2007

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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