Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing

Akira Maeda, Alan M. Zahler, Adrian R. Krainer, Mark B. Roth

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5′ splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.

Original languageEnglish
Pages (from-to)1301-1304
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number4
DOIs
Publication statusPublished - 15-02-1992

Fingerprint

Phosphoproteins
RNA Precursors
Nuclear Family
Drosophila
RNA Splice Sites
Cell-Free System
Alternative Splicing
Monoclonal Antibodies
RNA Polymerase II
Amphibians
Invertebrates
Serine
Arginine
Vertebrates
Catalytic Domain
Complementary DNA
Cell Line
Peptides
RNA Splicing Factors

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{d6668023b80343c7a19df593fa82f286,
title = "Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing",
abstract = "Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5′ splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.",
author = "Akira Maeda and Zahler, {Alan M.} and Krainer, {Adrian R.} and Roth, {Mark B.}",
year = "1992",
month = "2",
day = "15",
doi = "10.1073/pnas.89.4.1301",
language = "English",
volume = "89",
pages = "1301--1304",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing. / Maeda, Akira; Zahler, Alan M.; Krainer, Adrian R.; Roth, Mark B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 4, 15.02.1992, p. 1301-1304.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing

AU - Maeda, Akira

AU - Zahler, Alan M.

AU - Krainer, Adrian R.

AU - Roth, Mark B.

PY - 1992/2/15

Y1 - 1992/2/15

N2 - Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5′ splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.

AB - Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5′ splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.

UR - http://www.scopus.com/inward/record.url?scp=0026516573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026516573&partnerID=8YFLogxK

U2 - 10.1073/pnas.89.4.1301

DO - 10.1073/pnas.89.4.1301

M3 - Article

C2 - 1741384

AN - SCOPUS:0026516573

VL - 89

SP - 1301

EP - 1304

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -