Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice

Aiko Ishiwata; Misa Ozawa; Hiroshi Nagasaki; Makio Kato; Yusaku Noda; Takahiro Yamaguchi; Misuzu Nosaka; Sae Shimizu-Sato; Akie Nagasaki; Masahiko Maekawa; Hiro Yuki Hirano; Yutaka Sato

doi:10.1093/pcp/pct032

Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice

Aiko Ishiwata, Misa Ozawa, Hiroshi Nagasaki, Makio Kato, Yusaku Noda, Takahiro Yamaguchi, Misuzu Nosaka, Sae Shimizu-Sato, Akie Nagasaki, Masahiko Maekawa, Hiro Yuki Hirano, Yutaka Sato

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.

Original language	English
Pages (from-to)	779-792
Number of pages	14
Journal	Plant and Cell Physiology
Volume	54
Issue number	5
DOIs	https://doi.org/10.1093/pcp/pct032
Publication status	Published - 05-2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pct032

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Ishiwata, Aiko ; Ozawa, Misa ; Nagasaki, Hiroshi ; Kato, Makio ; Noda, Yusaku ; Yamaguchi, Takahiro ; Nosaka, Misuzu ; Shimizu-Sato, Sae ; Nagasaki, Akie ; Maekawa, Masahiko ; Hirano, Hiro Yuki ; Sato, Yutaka. / Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice. In: Plant and Cell Physiology. 2013 ; Vol. 54, No. 5. pp. 779-792.

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title = "Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice",

abstract = "Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.",

author = "Aiko Ishiwata and Misa Ozawa and Hiroshi Nagasaki and Makio Kato and Yusaku Noda and Takahiro Yamaguchi and Misuzu Nosaka and Sae Shimizu-Sato and Akie Nagasaki and Masahiko Maekawa and Hirano, {Hiro Yuki} and Yutaka Sato",

note = "Funding Information: This work was supported by the Ministry of Education, Science, Sports, and Culture of Japan [a Grant-in-Aid for Scientific Research (grant #21027017) to Y.S.].",

year = "2013",

month = may,

doi = "10.1093/pcp/pct032",

language = "English",

volume = "54",

pages = "779--792",

journal = "Plant and Cell Physiology",

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Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice. / Ishiwata, Aiko; Ozawa, Misa; Nagasaki, Hiroshi; Kato, Makio; Noda, Yusaku; Yamaguchi, Takahiro; Nosaka, Misuzu; Shimizu-Sato, Sae; Nagasaki, Akie; Maekawa, Masahiko; Hirano, Hiro Yuki; Sato, Yutaka.

In: Plant and Cell Physiology, Vol. 54, No. 5, 05.2013, p. 779-792.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice

AU - Ishiwata, Aiko

AU - Ozawa, Misa

AU - Nagasaki, Hiroshi

AU - Kato, Makio

AU - Noda, Yusaku

AU - Yamaguchi, Takahiro

AU - Nosaka, Misuzu

AU - Shimizu-Sato, Sae

AU - Nagasaki, Akie

AU - Maekawa, Masahiko

AU - Hirano, Hiro Yuki

AU - Sato, Yutaka

N1 - Funding Information: This work was supported by the Ministry of Education, Science, Sports, and Culture of Japan [a Grant-in-Aid for Scientific Research (grant #21027017) to Y.S.].

PY - 2013/5

Y1 - 2013/5

N2 - Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.

AB - Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.

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JF - Plant and Cell Physiology

SN - 0032-0781

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Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice

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