Type-II collagen gene expression is transiently upregulated in experimentally induced degeneration of rabbit intervertebral disc

H. Takaishi, O. Nemoto, M. Shiota, T. Kikuchi, Harumoto Yamada, M. Yamagishi, Y. Yabe

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

To clarify phenotypic alterations of intervertebral disc cells during the repair process, we cloned partial type-II collagen cDNA from rabbits and analyzed the level of expression of type-II collagen mRNA in disc degeneration. An animal model was created by surgical denucleation of rabbit intervertebral discs through an extraperitoneal approach. Eight animals each from an experimental and a control group were killed at 2, 4, 8, or 16 weeks postoperatively, and the disc samples were used for this study. Round chondrocyte-like cells that filled the herniated space showed intense signal of type-II collagen mRNA and significant pericellular immunostaining of type- II collagen but no clear staining of type-I collagen. Northern blot analysis revealed that the expression of type-II collagen mRNA of the repair disc cells was transiently increased at 4 weeks postoperatively. The cells were able to change their morphology in response to mechanical stimulation by surgical denucleation and to induce a significant increase in the gene expression of type-II collagen at an early phase of disc degeneration. The present results indicate the transient enhancement of repair activity in the degenerative process of injured fibrocartilage.

Original languageEnglish
Pages (from-to)528-538
Number of pages11
JournalJournal of Orthopaedic Research
Volume15
Issue number4
DOIs
Publication statusPublished - 01-07-1997
Externally publishedYes

Fingerprint

Intervertebral Disc Degeneration
Collagen Type II
Rabbits
Gene Expression
Intervertebral Disc
Messenger RNA
Fibrocartilage
Chondrocytes
Collagen Type I
Northern Blotting
Animal Models
Complementary DNA
Staining and Labeling
Control Groups

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Takaishi, H. ; Nemoto, O. ; Shiota, M. ; Kikuchi, T. ; Yamada, Harumoto ; Yamagishi, M. ; Yabe, Y. / Type-II collagen gene expression is transiently upregulated in experimentally induced degeneration of rabbit intervertebral disc. In: Journal of Orthopaedic Research. 1997 ; Vol. 15, No. 4. pp. 528-538.
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Type-II collagen gene expression is transiently upregulated in experimentally induced degeneration of rabbit intervertebral disc. / Takaishi, H.; Nemoto, O.; Shiota, M.; Kikuchi, T.; Yamada, Harumoto; Yamagishi, M.; Yabe, Y.

In: Journal of Orthopaedic Research, Vol. 15, No. 4, 01.07.1997, p. 528-538.

Research output: Contribution to journalArticle

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