Type II collagen synthesis in the articular cartilage of a rabbit model of osteoarthritis

Expression of type II collagen C-propeptide and mRNA especially during early-stage osteoarthritis

Hiraku Hotta, Harumoto Yamada, Hironari Takaishi, Tomoyuki Abe, Hideo Morioka, Toshiyuki Kikuchi, Kyosuke Fujikawa, Yoshiaki Toyama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. The aim of this study was to observe time course changes in type II collagen synthesis in various regions of articular cartilage affected with osteoarthritis (OA) by examining the expression of type II collagen C-propeptide (pCOL II-C) and mRNA in a rabbit OA model. Methods. Osteoarthritis was experimentally induced by partial lateral meniscectomy in the knees of Japanese white rabbits. The cartilage of the animals was then examined histologically over time. The degenerative area of articular cartilage was divided into three areas, according to the degree of degeneration. The ability to synthesize type II collagen was estimated by the immunohistological staining of pCOL II-C and the in situ hybridization of mRNA in type II collagen. Results. The positive rate of pCOL II-C immunostaining in chondrocytes was highest in the central-degenerative region 1 week after surgery, and the highest rate in the para-degenerative region was observed 2 and 4 weeks after surgery. The percentage of pCOL II-C positive cells increased as the histological degeneration score increased to moderate degeneration and then decreased with further progression of the severity of cartilage degeneration. Examination by in situ hybridization revealed that the regions marked by strong pCOL II-C mRNA expression were similar to those indicated by the immunohistology results. Conclusions. These results suggest that the type II collagen-synthesizing potential of chondrocytes is highest in moderately degenerated areas of OA articular cartilage. Cartilage repair continues to be seen even as OA advances, although the reaction varies depending on the stage of OA.

Original languageEnglish
Pages (from-to)595-607
Number of pages13
JournalJournal of Orthopaedic Science
Volume10
Issue number6
DOIs
Publication statusPublished - 01-01-2005

Fingerprint

Collagen Type II
Articular Cartilage
Osteoarthritis
Rabbits
Messenger RNA
Cartilage
Chondrocytes
In Situ Hybridization
chondrocalcin
Knee
procollagen type II carboxy-terminal peptide
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Hotta, Hiraku ; Yamada, Harumoto ; Takaishi, Hironari ; Abe, Tomoyuki ; Morioka, Hideo ; Kikuchi, Toshiyuki ; Fujikawa, Kyosuke ; Toyama, Yoshiaki. / Type II collagen synthesis in the articular cartilage of a rabbit model of osteoarthritis : Expression of type II collagen C-propeptide and mRNA especially during early-stage osteoarthritis. In: Journal of Orthopaedic Science. 2005 ; Vol. 10, No. 6. pp. 595-607.
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abstract = "Background. The aim of this study was to observe time course changes in type II collagen synthesis in various regions of articular cartilage affected with osteoarthritis (OA) by examining the expression of type II collagen C-propeptide (pCOL II-C) and mRNA in a rabbit OA model. Methods. Osteoarthritis was experimentally induced by partial lateral meniscectomy in the knees of Japanese white rabbits. The cartilage of the animals was then examined histologically over time. The degenerative area of articular cartilage was divided into three areas, according to the degree of degeneration. The ability to synthesize type II collagen was estimated by the immunohistological staining of pCOL II-C and the in situ hybridization of mRNA in type II collagen. Results. The positive rate of pCOL II-C immunostaining in chondrocytes was highest in the central-degenerative region 1 week after surgery, and the highest rate in the para-degenerative region was observed 2 and 4 weeks after surgery. The percentage of pCOL II-C positive cells increased as the histological degeneration score increased to moderate degeneration and then decreased with further progression of the severity of cartilage degeneration. Examination by in situ hybridization revealed that the regions marked by strong pCOL II-C mRNA expression were similar to those indicated by the immunohistology results. Conclusions. These results suggest that the type II collagen-synthesizing potential of chondrocytes is highest in moderately degenerated areas of OA articular cartilage. Cartilage repair continues to be seen even as OA advances, although the reaction varies depending on the stage of OA.",
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Type II collagen synthesis in the articular cartilage of a rabbit model of osteoarthritis : Expression of type II collagen C-propeptide and mRNA especially during early-stage osteoarthritis. / Hotta, Hiraku; Yamada, Harumoto; Takaishi, Hironari; Abe, Tomoyuki; Morioka, Hideo; Kikuchi, Toshiyuki; Fujikawa, Kyosuke; Toyama, Yoshiaki.

In: Journal of Orthopaedic Science, Vol. 10, No. 6, 01.01.2005, p. 595-607.

Research output: Contribution to journalArticle

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T1 - Type II collagen synthesis in the articular cartilage of a rabbit model of osteoarthritis

T2 - Expression of type II collagen C-propeptide and mRNA especially during early-stage osteoarthritis

AU - Hotta, Hiraku

AU - Yamada, Harumoto

AU - Takaishi, Hironari

AU - Abe, Tomoyuki

AU - Morioka, Hideo

AU - Kikuchi, Toshiyuki

AU - Fujikawa, Kyosuke

AU - Toyama, Yoshiaki

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Y1 - 2005/1/1

N2 - Background. The aim of this study was to observe time course changes in type II collagen synthesis in various regions of articular cartilage affected with osteoarthritis (OA) by examining the expression of type II collagen C-propeptide (pCOL II-C) and mRNA in a rabbit OA model. Methods. Osteoarthritis was experimentally induced by partial lateral meniscectomy in the knees of Japanese white rabbits. The cartilage of the animals was then examined histologically over time. The degenerative area of articular cartilage was divided into three areas, according to the degree of degeneration. The ability to synthesize type II collagen was estimated by the immunohistological staining of pCOL II-C and the in situ hybridization of mRNA in type II collagen. Results. The positive rate of pCOL II-C immunostaining in chondrocytes was highest in the central-degenerative region 1 week after surgery, and the highest rate in the para-degenerative region was observed 2 and 4 weeks after surgery. The percentage of pCOL II-C positive cells increased as the histological degeneration score increased to moderate degeneration and then decreased with further progression of the severity of cartilage degeneration. Examination by in situ hybridization revealed that the regions marked by strong pCOL II-C mRNA expression were similar to those indicated by the immunohistology results. Conclusions. These results suggest that the type II collagen-synthesizing potential of chondrocytes is highest in moderately degenerated areas of OA articular cartilage. Cartilage repair continues to be seen even as OA advances, although the reaction varies depending on the stage of OA.

AB - Background. The aim of this study was to observe time course changes in type II collagen synthesis in various regions of articular cartilage affected with osteoarthritis (OA) by examining the expression of type II collagen C-propeptide (pCOL II-C) and mRNA in a rabbit OA model. Methods. Osteoarthritis was experimentally induced by partial lateral meniscectomy in the knees of Japanese white rabbits. The cartilage of the animals was then examined histologically over time. The degenerative area of articular cartilage was divided into three areas, according to the degree of degeneration. The ability to synthesize type II collagen was estimated by the immunohistological staining of pCOL II-C and the in situ hybridization of mRNA in type II collagen. Results. The positive rate of pCOL II-C immunostaining in chondrocytes was highest in the central-degenerative region 1 week after surgery, and the highest rate in the para-degenerative region was observed 2 and 4 weeks after surgery. The percentage of pCOL II-C positive cells increased as the histological degeneration score increased to moderate degeneration and then decreased with further progression of the severity of cartilage degeneration. Examination by in situ hybridization revealed that the regions marked by strong pCOL II-C mRNA expression were similar to those indicated by the immunohistology results. Conclusions. These results suggest that the type II collagen-synthesizing potential of chondrocytes is highest in moderately degenerated areas of OA articular cartilage. Cartilage repair continues to be seen even as OA advances, although the reaction varies depending on the stage of OA.

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