Tyrosine phosphorylation of caveolin-1 in the endothelium

Takeo Aoki, Ryuji Nomura, Toyoshi Fujimoto

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Caveolin-1, a scaffolding protein of caveolae, is known to be tyrosine- phosphorylated by Src kinases. Recently we generated a specific antibody to caveolin-1 phosphorylated at tyrosine. 14 (PY14) (R. Nomura and T. Fujimoto, 1999, Mol. Biol. Cell 10, 975-986). In the present study, by applying PY14 to sections of normal rat tissues, we found that tyrosine phosphorylation of caveolin-1 occurred in limited locations, including the endothelium of the continuous capillaries and small venules. Cultured endothelial cells were not labeled by PY14 under a standard culture condition, but became positively labeled when exposed to oxidative stresses and/or tyrosine phosphatase inhibitors. The reaction was prohibited by pretreating the cells with herbimycin A or genistein. Vasoactive reagents or physical stimuli did not cause the phosphorylation. Concomitant with the tyrosine phosphorylation, the number of invaginated caveolae decreased drastically, and vesicles labeled intensely for caveolin-1 appeared in the cytoplasm; the average diameter of the vesicles was larger than that of caveolae. The result implies that tyrosine phosphorylation of caveolin-1 occurs at tyrosine-14 in the normal rat endothelium in vivo and may induce caveolar vesiculation and/or fusion.

Original languageEnglish
Pages (from-to)629-636
Number of pages8
JournalExperimental Cell Research
Volume253
Issue number2
DOIs
Publication statusPublished - 15-12-1999

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Caveolin 1
Endothelium
Tyrosine
Phosphorylation
Caveolae
Venules
src-Family Kinases
Genistein
Vascular Endothelium
Phosphoric Monoester Hydrolases
Cultured Cells
Cytoplasm
Oxidative Stress
Endothelial Cells
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Aoki, Takeo ; Nomura, Ryuji ; Fujimoto, Toyoshi. / Tyrosine phosphorylation of caveolin-1 in the endothelium. In: Experimental Cell Research. 1999 ; Vol. 253, No. 2. pp. 629-636.
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Tyrosine phosphorylation of caveolin-1 in the endothelium. / Aoki, Takeo; Nomura, Ryuji; Fujimoto, Toyoshi.

In: Experimental Cell Research, Vol. 253, No. 2, 15.12.1999, p. 629-636.

Research output: Contribution to journalArticle

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