U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

Kazuhiro Fukumura, Ichiro Taniguchi, Hiroshi Sakamoto, Mutsuhito Ohno, Kunio Inoue

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

Original languageEnglish
Pages (from-to)1907-1914
Number of pages8
JournalNucleic Acids Research
Volume37
Issue number6
DOIs
Publication statusPublished - 21-04-2009

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RNA Precursors
Alternative Splicing
Introns
U1 Small Nuclear Ribonucleoproteins
RNA Splice Sites
Xenopus
Oocytes
Exons

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Fukumura, Kazuhiro ; Taniguchi, Ichiro ; Sakamoto, Hiroshi ; Ohno, Mutsuhito ; Inoue, Kunio. / U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing. In: Nucleic Acids Research. 2009 ; Vol. 37, No. 6. pp. 1907-1914.
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U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing. / Fukumura, Kazuhiro; Taniguchi, Ichiro; Sakamoto, Hiroshi; Ohno, Mutsuhito; Inoue, Kunio.

In: Nucleic Acids Research, Vol. 37, No. 6, 21.04.2009, p. 1907-1914.

Research output: Contribution to journalArticle

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