U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

Kazuhiro Fukumura, Ichiro Taniguchi, Hiroshi Sakamoto, Mutsuhito Ohno, Kunio Inoue

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23 Citations (Scopus)

Abstract

U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

Original languageEnglish
Pages (from-to)1907-1914
Number of pages8
JournalNucleic Acids Research
Volume37
Issue number6
DOIs
Publication statusPublished - 21-04-2009

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All Science Journal Classification (ASJC) codes

  • Genetics

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