U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

Kazuhiro Fukumura; Ichiro Taniguchi; Hiroshi Sakamoto; Mutsuhito Ohno; Kunio Inoue

doi:10.1093/nar/gkp050

U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

Kazuhiro Fukumura, Ichiro Taniguchi, Hiroshi Sakamoto, Mutsuhito Ohno, Kunio Inoue

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

Original language	English
Pages (from-to)	1907-1914
Number of pages	8
Journal	Nucleic acids research
Volume	37
Issue number	6
DOIs	https://doi.org/10.1093/nar/gkp050
Publication status	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1093/nar/gkp050

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title = "U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing",

abstract = "U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.",

author = "Kazuhiro Fukumura and Ichiro Taniguchi and Hiroshi Sakamoto and Mutsuhito Ohno and Kunio Inoue",

note = "Funding Information: Grants-in-Aid for Scientific Research from JSPS and MEXT; and by the Mitsubishi Foundation, partial. Funding for open access charge: Mitsubishi Foundation.",

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T1 - U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

AU - Fukumura, Kazuhiro

AU - Taniguchi, Ichiro

AU - Sakamoto, Hiroshi

AU - Ohno, Mutsuhito

AU - Inoue, Kunio

N1 - Funding Information: Grants-in-Aid for Scientific Research from JSPS and MEXT; and by the Mitsubishi Foundation, partial. Funding for open access charge: Mitsubishi Foundation.

PY - 2009

Y1 - 2009

N2 - U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

AB - U1 snRNP plays a crucial role in the 5′ splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1γ (hF1γ) intron 9 in vitro. Moreover, hF1γ intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1γ exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

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U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing

Abstract

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