Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)

Kazue Okubo; Keisuke Yamano; Qiwei Qin; Kazuhiko Aoyagi; Mitsuru Ototake; Teruyuki Nakanishi; Hideo Fukuda; Johannes M. Dijkstra

doi:10.1006/fsim.2001.0375

Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)

Kazue Okubo, Keisuke Yamano, Qiwei Qin, Kazuhiko Aoyagi, Mitsuru Ototake, Teruyuki Nakanishi, Hideo Fukuda, Johannes M. Dijkstra

Division of Biomedical Polymer Science

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.

Original language	English
Pages (from-to)	335-351
Number of pages	17
Journal	Fish and Shellfish Immunology
Volume	12
Issue number	4
DOIs	https://doi.org/10.1006/fsim.2001.0375
Publication status	Published - 01-01-2002

Fingerprint

All Science Journal Classification (ASJC) codes

Environmental Chemistry
Aquatic Science

Cite this

Okubo, K., Yamano, K., Qin, Q., Aoyagi, K., Ototake, M., Nakanishi, T., Fukuda, H., & Dijkstra, J. M. (2002). Ubiquitin genes in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 12(4), 335-351. https://doi.org/10.1006/fsim.2001.0375

@article{5e0a37a83681405b881a27e49b99af75,

title = "Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)",

abstract = "Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.",

author = "Kazue Okubo and Keisuke Yamano and Qiwei Qin and Kazuhiko Aoyagi and Mitsuru Ototake and Teruyuki Nakanishi and Hideo Fukuda and Dijkstra, {Johannes M.}",

year = "2002",

month = jan

day = "1",

doi = "10.1006/fsim.2001.0375",

language = "English",

volume = "12",

pages = "335--351",

journal = "Fish and Shellfish Immunology",

issn = "1050-4648",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)

AU - Okubo, Kazue

AU - Yamano, Keisuke

AU - Qin, Qiwei

AU - Aoyagi, Kazuhiko

AU - Ototake, Mitsuru

AU - Nakanishi, Teruyuki

AU - Fukuda, Hideo

AU - Dijkstra, Johannes M.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.

AB - Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.

UR - http://www.scopus.com/inward/record.url?scp=0036012804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036012804&partnerID=8YFLogxK

U2 - 10.1006/fsim.2001.0375

DO - 10.1006/fsim.2001.0375

M3 - Article

C2 - 12049169

AN - SCOPUS:0036012804

VL - 12

SP - 335

EP - 351

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 4

ER -

Access to Document

10.1006/fsim.2001.0375