Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)

Kazue Okubo, Keisuke Yamano, Qiwei Qin, Kazuhiko Aoyagi, Mitsuru Ototake, Teruyuki Nakanishi, Hideo Fukuda, Johannes M. Dijkstra

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Abstract

Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.

Original languageEnglish
Pages (from-to)335-351
Number of pages17
JournalFish and Shellfish Immunology
Volume12
Issue number4
DOIs
Publication statusPublished - 01-01-2002

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All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Okubo, K., Yamano, K., Qin, Q., Aoyagi, K., Ototake, M., Nakanishi, T., Fukuda, H., & Dijkstra, J. M. (2002). Ubiquitin genes in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 12(4), 335-351. https://doi.org/10.1006/fsim.2001.0375