TY - JOUR
T1 - Ubiquitin genes in rainbow trout (Oncorhynchus mykiss)
AU - Okubo, Kazue
AU - Yamano, Keisuke
AU - Qin, Qiwei
AU - Aoyagi, Kazuhiko
AU - Ototake, Mitsuru
AU - Nakanishi, Teruyuki
AU - Fukuda, Hideo
AU - Dijkstra, Johannes M.
N1 - Funding Information:
This study was supported by ‘the promotion of basic research activities for innovative biosciences’ funded by Bio-oriented Technology Research Advancement Institution (BRAIN) and supported by a post-doctoral fellowship for Qiwei Qin from the Science and Technology Agency of Japan. We thank Mr Soichiro Hayashi from the Hayashi trout farm in Fukushima, Japan, for kindly giving us the fish used in this study. We thank Dr James D. Moore, Bodega Marine Laboratory, U.S.A., for his help in editing this article.
PY - 2002
Y1 - 2002
N2 - Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.
AB - Ubiquitin is a small protein involved in intracellular proteolysis. It is highly conserved throughout eukaryotic phyla and has been detected in such diverse species as yeast, barley, Drosophila and man. A previous study showed that chromatin of rainbow trout testis contains free ubiquitin with a sequence similar to that of other phyla. In the present study, which focused on rainbow trout but included eleven other species, it is shown that fish ubiquitin genetic organisation and expression are similar to those of other phylogenetic groups through the following set of observations: (a) Multiple loci were detected, (b) These loci encode repeats of ubiquitin, (c) Although the DNA sequences are not conserved, the encoded amino acid sequences are fully conserved, (d) The expression of ubiquitin was influenced by cell culture conditions and viral infection.
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U2 - 10.1006/fsim.2001.0375
DO - 10.1006/fsim.2001.0375
M3 - Article
C2 - 12049169
AN - SCOPUS:0036012804
SN - 1050-4648
VL - 12
SP - 335
EP - 351
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 4
ER -