Ubiquitination and proteasome-dependent degradation of human eukaryotic translation initiation factor 4E

Takayuki Murata, Kunitada Shimotohno

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Translation initiation factor 4E (eIF4E) is a cytoplasmic cap-binding protein that is required for cap-dependent translation initiation. Here, we have shown that eIF4E is ubiquitinated primarily at Lys-159 and incubation of cells with a proteasome inhibitor leads to increased eIF4E levels, suggesting the proteasome-dependent proteolysis of ubiquitinated eIF4E. Ubiquitinated eIF4E retained its cap binding ability, whereas eIF4E phosphorylation and eIF4G binding were reduced by ubiquitination. The W73A mutant of eIF4E exhibited enhanced ubiquitination/degradation, and 4E-BP overexpression protected eIF4E from ubiquitination/degradation. Because heat shock or the expression of the carboxyl terminus of heat shock cognate protein 70-interacting protein (Chip) dramatically increased eIF4E ubiquitination, Chip may be at least one ubiquitin E3 ligase responsible for eIF4E ubiquitination.

Original languageEnglish
Pages (from-to)20788-20800
Number of pages13
JournalJournal of Biological Chemistry
Volume281
Issue number30
DOIs
Publication statusPublished - 28-07-2006

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Eukaryotic Initiation Factor-4E
Eukaryotic Initiation Factors
Ubiquitination
Proteasome Endopeptidase Complex
RNA Cap-Binding Proteins
HSC70 Heat-Shock Proteins
Proteolysis
Degradation
Peptide Initiation Factors
Phosphorylation
Proteasome Inhibitors
Ubiquitin-Protein Ligases
Cells
Protein Array Analysis
Proteins
Shock
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Ubiquitination and proteasome-dependent degradation of human eukaryotic translation initiation factor 4E. / Murata, Takayuki; Shimotohno, Kunitada.

In: Journal of Biological Chemistry, Vol. 281, No. 30, 28.07.2006, p. 20788-20800.

Research output: Contribution to journalArticle

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