Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

Yoshimasa Maeno, Hisashi Fujioka, Michael R. Hollingdale, Christian F. Ockenhouse, Shusuke Nakazawa, Masamichi Aikawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

Original languageEnglish
Article number71100
Pages (from-to)383-390
Number of pages8
JournalExperimental Parasitology
Volume79
Issue number3
DOIs
Publication statusPublished - 01-01-1994
Externally publishedYes

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Amelanotic Melanoma
Hep G2 Cells
Hepatocytes
Hepatocellular Carcinoma
Liver
Microvilli
Endothelial Cells
Sporozoites
Malaria
Immunohistochemistry
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Immunology
  • Infectious Diseases

Cite this

Maeno, Yoshimasa ; Fujioka, Hisashi ; Hollingdale, Michael R. ; Ockenhouse, Christian F. ; Nakazawa, Shusuke ; Aikawa, Masamichi. / Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells. In: Experimental Parasitology. 1994 ; Vol. 79, No. 3. pp. 383-390.
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abstract = "CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.",
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Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells. / Maeno, Yoshimasa; Fujioka, Hisashi; Hollingdale, Michael R.; Ockenhouse, Christian F.; Nakazawa, Shusuke; Aikawa, Masamichi.

In: Experimental Parasitology, Vol. 79, No. 3, 71100, 01.01.1994, p. 383-390.

Research output: Contribution to journalArticle

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