Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

Yoshimasa Maeno; Hisashi Fujioka; Michael R. Hollingdale; Christian F. Ockenhouse; Shusuke Nakazawa; Masamichi Aikawa

doi:10.1006/expr.1994.1100

Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

Yoshimasa Maeno, Hisashi Fujioka, Michael R. Hollingdale, Christian F. Ockenhouse, Shusuke Nakazawa, Masamichi Aikawa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

Original language	English
Article number	71100
Pages (from-to)	383-390
Number of pages	8
Journal	Experimental Parasitology
Volume	79
Issue number	3
DOIs	https://doi.org/10.1006/expr.1994.1100
Publication status	Published - 11-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Parasitology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/expr.1994.1100

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title = "Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells",

abstract = "CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.",

author = "Yoshimasa Maeno and Hisashi Fujioka and Hollingdale, {Michael R.} and Ockenhouse, {Christian F.} and Shusuke Nakazawa and Masamichi Aikawa",

year = "1994",

month = nov,

doi = "10.1006/expr.1994.1100",

language = "English",

volume = "79",

pages = "383--390",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "3",

}

Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells. / Maeno, Yoshimasa; Fujioka, Hisashi; Hollingdale, Michael R. et al.
In: Experimental Parasitology, Vol. 79, No. 3, 71100, 11.1994, p. 383-390.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

AU - Maeno, Yoshimasa

AU - Fujioka, Hisashi

AU - Hollingdale, Michael R.

AU - Ockenhouse, Christian F.

AU - Nakazawa, Shusuke

AU - Aikawa, Masamichi

PY - 1994/11

Y1 - 1994/11

N2 - CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

AB - CD36 is expressed in the endothelial cells of some human organs, but the ultrastructural localization of this molecule in the sinusoidal lining cells of human liver is not well established. We report the ultrastructural localization of CD36 in the liver using a novel murine monoclonal antibody against CD36, namely MO30, as a primary antibody. Immunocytochemistry by the postembedding method showed that CD36 was localized in endothelial cells of sinusoids and in hepatocyte microvilli protruding into the space of Disse. Moreover, in cultured human hepatoma (HepG2-A16) cells and C32 amelanotic melanoma cells, MO30 reacted with microvilli. Hence, CD36 expressed on these cells may be involved in recognition and/or entry of these cells by malaria sporozoites.

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DO - 10.1006/expr.1994.1100

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SN - 0014-4894

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JO - Experimental Parasitology

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ER -

Ultrastructural localization of CD36 in human hepatic sinusoidal lining cells, hepatocytes, human hepatoma (HepG2-A16) cells, and C32 amelanotic melanoma cells

Abstract

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UN SDGs

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