Abstract
Background: Although changes in the fine balance of allergen-specific T cells are crucial in the pathogenesis of allergic diseases, their roles in the allergic reaction to hen's eggs (HE) have not yet been fully analysed. Objective: Using microarray technology, allergen-stimulated T cells from HE-allergic children were analysed to identify genes that are specifically up-regulated in these cells. Methods: RNA from CD4+ CD14- cells, fractionated from allergen-stimulated peripheral mononuclear cells, was analysed using a whole -genome microarray and real-time RT-PCR. The protein expression of selected genes was ascertained by flow cytometry. Results: In microarray analyses of allergen-stimulated T cells, 43 genes were up-regulated in HE-allergic children but not in non-HE-allergic children. Among these, up-regulation of three genes, cytokine -inducible SH2-containing protein (CISH), nuclear factor of κ light polypeptide gene enhancer in B-cell inhibitor Z (NFKBIZ) and B-cell CLL/lymphoma 2 (BCL2), was confirmed by real-time quantitative RT-PCR. CISH, but not NFKBIZ or BCL2, showed a significantly higher ratio of antigen-stimulated cell transcription over unstimulated cells in HE-allergic than in non-HE-allergic children (P<0.01). Flow-cytometric analysis revealed that the percentage of CD25+CISH+ cells in CD4+ cells from patients with HE allergy was significantly higher than that in controls (P<0.01). The expression level of CISH was significantly higher in IL-4+ Th2 cells than in IFN- γ+ Th1 cells. Conclusion: We noted that CISH expression in allergen-stimulated CD4+ T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children.
| Original language | English |
|---|---|
| Pages (from-to) | 1499-1506 |
| Number of pages | 8 |
| Journal | Clinical and Experimental Allergy |
| Volume | 38 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 01-09-2008 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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Up-regulated cytokine-inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients. / Nakajima, Yoichi; Tsuge, I.; Kondo, Y.; Komatsubara, R.; Hirata, N.; Kakami, M.; Kato, Maki; Kurahashi, Hiroki; Urisu, A.; Asano, Y.
In: Clinical and Experimental Allergy, Vol. 38, No. 9, 01.09.2008, p. 1499-1506.Research output: Contribution to journal › Article
TY - JOUR
T1 - Up-regulated cytokine-inducible SH2-containing protein expression in allergen-stimulated T cells from hen's egg-allergic patients
AU - Nakajima, Yoichi
AU - Tsuge, I.
AU - Kondo, Y.
AU - Komatsubara, R.
AU - Hirata, N.
AU - Kakami, M.
AU - Kato, Maki
AU - Kurahashi, Hiroki
AU - Urisu, A.
AU - Asano, Y.
PY - 2008/9/1
Y1 - 2008/9/1
N2 - Background: Although changes in the fine balance of allergen-specific T cells are crucial in the pathogenesis of allergic diseases, their roles in the allergic reaction to hen's eggs (HE) have not yet been fully analysed. Objective: Using microarray technology, allergen-stimulated T cells from HE-allergic children were analysed to identify genes that are specifically up-regulated in these cells. Methods: RNA from CD4+ CD14- cells, fractionated from allergen-stimulated peripheral mononuclear cells, was analysed using a whole -genome microarray and real-time RT-PCR. The protein expression of selected genes was ascertained by flow cytometry. Results: In microarray analyses of allergen-stimulated T cells, 43 genes were up-regulated in HE-allergic children but not in non-HE-allergic children. Among these, up-regulation of three genes, cytokine -inducible SH2-containing protein (CISH), nuclear factor of κ light polypeptide gene enhancer in B-cell inhibitor Z (NFKBIZ) and B-cell CLL/lymphoma 2 (BCL2), was confirmed by real-time quantitative RT-PCR. CISH, but not NFKBIZ or BCL2, showed a significantly higher ratio of antigen-stimulated cell transcription over unstimulated cells in HE-allergic than in non-HE-allergic children (P<0.01). Flow-cytometric analysis revealed that the percentage of CD25+CISH+ cells in CD4+ cells from patients with HE allergy was significantly higher than that in controls (P<0.01). The expression level of CISH was significantly higher in IL-4+ Th2 cells than in IFN- γ+ Th1 cells. Conclusion: We noted that CISH expression in allergen-stimulated CD4+ T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children.
AB - Background: Although changes in the fine balance of allergen-specific T cells are crucial in the pathogenesis of allergic diseases, their roles in the allergic reaction to hen's eggs (HE) have not yet been fully analysed. Objective: Using microarray technology, allergen-stimulated T cells from HE-allergic children were analysed to identify genes that are specifically up-regulated in these cells. Methods: RNA from CD4+ CD14- cells, fractionated from allergen-stimulated peripheral mononuclear cells, was analysed using a whole -genome microarray and real-time RT-PCR. The protein expression of selected genes was ascertained by flow cytometry. Results: In microarray analyses of allergen-stimulated T cells, 43 genes were up-regulated in HE-allergic children but not in non-HE-allergic children. Among these, up-regulation of three genes, cytokine -inducible SH2-containing protein (CISH), nuclear factor of κ light polypeptide gene enhancer in B-cell inhibitor Z (NFKBIZ) and B-cell CLL/lymphoma 2 (BCL2), was confirmed by real-time quantitative RT-PCR. CISH, but not NFKBIZ or BCL2, showed a significantly higher ratio of antigen-stimulated cell transcription over unstimulated cells in HE-allergic than in non-HE-allergic children (P<0.01). Flow-cytometric analysis revealed that the percentage of CD25+CISH+ cells in CD4+ cells from patients with HE allergy was significantly higher than that in controls (P<0.01). The expression level of CISH was significantly higher in IL-4+ Th2 cells than in IFN- γ+ Th1 cells. Conclusion: We noted that CISH expression in allergen-stimulated CD4+ T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children.
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UR - http://www.scopus.com/inward/citedby.url?scp=50249129285&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2222.2008.03030.x
DO - 10.1111/j.1365-2222.2008.03030.x
M3 - Article
C2 - 18647318
AN - SCOPUS:50249129285
VL - 38
SP - 1499
EP - 1506
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
SN - 0954-7894
IS - 9
ER -