Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia

Chihiro Kakiuchi, Mizuho Ishiwata, Shinichiro Nanko, Norio Ozaki, Nakao Iwata, Tadashi Umekage, Mamoru Tochigi, Kazuhisa Kohda, Tsukasa Sasaki, Akira Imamura, Yuji Okazaki, Tadafumi Kato

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The contribution of genetic factors to schizophrenia is well established and recent studies have indicated several strong candidate genes. However, the pathophysiology of schizophrenia has not been totally elucidated yet. To date, studies of monozygotic twins discordant for schizophrenia have provided insight into the pathophysiology of this illness; this type of study can exclude inter-individual variability and confounding factors such as effects of drugs. In this study we used DNA microarray analysis to examine the mRNA expression patterns in the lymphoblastoid (LB) cells derived from two pairs of monozygotic twins discordant for schizophrenia. From five independent replicates for each pair of twins, we selected five genes, which included adrenomedullin (ADM) and selenoprotein X1 (SEPX1), as significantly changed in both twins with schizophrenia. Interestingly, ADM was previously reported to be up-regulated in both the LB cells and plasma of schizophrenic patients, and SEPX1 was included in the list of genes up-regulated in the peripheral blood cells of schizophrenia patients by microarray analysis. Then, we performed a genetic association study of schizophrenia in the Japanese population and examined the copy number variations, but observed no association. These findings suggest the possible role of ADM and SEPX1 as biomarkers of schizophrenia. The results also support the usefulness of gene expression analysis in LB cells of monozygotic twins discordant for an illness.

Original languageEnglish
Pages (from-to)557-564
Number of pages8
JournalAmerican Journal of Medical Genetics, Part B: Neuropsychiatric Genetics
Volume147
Issue number5
DOIs
Publication statusPublished - 05-07-2008

Fingerprint

Selenoproteins
Adrenomedullin
Monozygotic Twins
Schizophrenia
Up-Regulation
Microarray Analysis
Genes
Genetic Association Studies
Plasma Cells
Oligonucleotide Array Sequence Analysis
Blood Cells
Biomarkers
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Kakiuchi, Chihiro ; Ishiwata, Mizuho ; Nanko, Shinichiro ; Ozaki, Norio ; Iwata, Nakao ; Umekage, Tadashi ; Tochigi, Mamoru ; Kohda, Kazuhisa ; Sasaki, Tsukasa ; Imamura, Akira ; Okazaki, Yuji ; Kato, Tadafumi. / Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia. In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics. 2008 ; Vol. 147, No. 5. pp. 557-564.
@article{3b6456f3bafc4380ad6816e0ff6ce7d3,
title = "Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia",
abstract = "The contribution of genetic factors to schizophrenia is well established and recent studies have indicated several strong candidate genes. However, the pathophysiology of schizophrenia has not been totally elucidated yet. To date, studies of monozygotic twins discordant for schizophrenia have provided insight into the pathophysiology of this illness; this type of study can exclude inter-individual variability and confounding factors such as effects of drugs. In this study we used DNA microarray analysis to examine the mRNA expression patterns in the lymphoblastoid (LB) cells derived from two pairs of monozygotic twins discordant for schizophrenia. From five independent replicates for each pair of twins, we selected five genes, which included adrenomedullin (ADM) and selenoprotein X1 (SEPX1), as significantly changed in both twins with schizophrenia. Interestingly, ADM was previously reported to be up-regulated in both the LB cells and plasma of schizophrenic patients, and SEPX1 was included in the list of genes up-regulated in the peripheral blood cells of schizophrenia patients by microarray analysis. Then, we performed a genetic association study of schizophrenia in the Japanese population and examined the copy number variations, but observed no association. These findings suggest the possible role of ADM and SEPX1 as biomarkers of schizophrenia. The results also support the usefulness of gene expression analysis in LB cells of monozygotic twins discordant for an illness.",
author = "Chihiro Kakiuchi and Mizuho Ishiwata and Shinichiro Nanko and Norio Ozaki and Nakao Iwata and Tadashi Umekage and Mamoru Tochigi and Kazuhisa Kohda and Tsukasa Sasaki and Akira Imamura and Yuji Okazaki and Tadafumi Kato",
year = "2008",
month = "7",
day = "5",
doi = "10.1002/ajmg.b.30643",
language = "English",
volume = "147",
pages = "557--564",
journal = "American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics",
issn = "1552-4841",
publisher = "Wiley-Liss Inc.",
number = "5",

}

Kakiuchi, C, Ishiwata, M, Nanko, S, Ozaki, N, Iwata, N, Umekage, T, Tochigi, M, Kohda, K, Sasaki, T, Imamura, A, Okazaki, Y & Kato, T 2008, 'Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia', American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, vol. 147, no. 5, pp. 557-564. https://doi.org/10.1002/ajmg.b.30643

Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia. / Kakiuchi, Chihiro; Ishiwata, Mizuho; Nanko, Shinichiro; Ozaki, Norio; Iwata, Nakao; Umekage, Tadashi; Tochigi, Mamoru; Kohda, Kazuhisa; Sasaki, Tsukasa; Imamura, Akira; Okazaki, Yuji; Kato, Tadafumi.

In: American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics, Vol. 147, No. 5, 05.07.2008, p. 557-564.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia

AU - Kakiuchi, Chihiro

AU - Ishiwata, Mizuho

AU - Nanko, Shinichiro

AU - Ozaki, Norio

AU - Iwata, Nakao

AU - Umekage, Tadashi

AU - Tochigi, Mamoru

AU - Kohda, Kazuhisa

AU - Sasaki, Tsukasa

AU - Imamura, Akira

AU - Okazaki, Yuji

AU - Kato, Tadafumi

PY - 2008/7/5

Y1 - 2008/7/5

N2 - The contribution of genetic factors to schizophrenia is well established and recent studies have indicated several strong candidate genes. However, the pathophysiology of schizophrenia has not been totally elucidated yet. To date, studies of monozygotic twins discordant for schizophrenia have provided insight into the pathophysiology of this illness; this type of study can exclude inter-individual variability and confounding factors such as effects of drugs. In this study we used DNA microarray analysis to examine the mRNA expression patterns in the lymphoblastoid (LB) cells derived from two pairs of monozygotic twins discordant for schizophrenia. From five independent replicates for each pair of twins, we selected five genes, which included adrenomedullin (ADM) and selenoprotein X1 (SEPX1), as significantly changed in both twins with schizophrenia. Interestingly, ADM was previously reported to be up-regulated in both the LB cells and plasma of schizophrenic patients, and SEPX1 was included in the list of genes up-regulated in the peripheral blood cells of schizophrenia patients by microarray analysis. Then, we performed a genetic association study of schizophrenia in the Japanese population and examined the copy number variations, but observed no association. These findings suggest the possible role of ADM and SEPX1 as biomarkers of schizophrenia. The results also support the usefulness of gene expression analysis in LB cells of monozygotic twins discordant for an illness.

AB - The contribution of genetic factors to schizophrenia is well established and recent studies have indicated several strong candidate genes. However, the pathophysiology of schizophrenia has not been totally elucidated yet. To date, studies of monozygotic twins discordant for schizophrenia have provided insight into the pathophysiology of this illness; this type of study can exclude inter-individual variability and confounding factors such as effects of drugs. In this study we used DNA microarray analysis to examine the mRNA expression patterns in the lymphoblastoid (LB) cells derived from two pairs of monozygotic twins discordant for schizophrenia. From five independent replicates for each pair of twins, we selected five genes, which included adrenomedullin (ADM) and selenoprotein X1 (SEPX1), as significantly changed in both twins with schizophrenia. Interestingly, ADM was previously reported to be up-regulated in both the LB cells and plasma of schizophrenic patients, and SEPX1 was included in the list of genes up-regulated in the peripheral blood cells of schizophrenia patients by microarray analysis. Then, we performed a genetic association study of schizophrenia in the Japanese population and examined the copy number variations, but observed no association. These findings suggest the possible role of ADM and SEPX1 as biomarkers of schizophrenia. The results also support the usefulness of gene expression analysis in LB cells of monozygotic twins discordant for an illness.

UR - http://www.scopus.com/inward/record.url?scp=46949092428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46949092428&partnerID=8YFLogxK

U2 - 10.1002/ajmg.b.30643

DO - 10.1002/ajmg.b.30643

M3 - Article

C2 - 18081029

AN - SCOPUS:46949092428

VL - 147

SP - 557

EP - 564

JO - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

JF - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

SN - 1552-4841

IS - 5

ER -