TY - JOUR
T1 - Up-regulation of calcineurin aβ mRNA in the Alzheimer's disease brain
T2 - Assessment by cDNA microarray
AU - Hata, Ryuji
AU - Masumura, Makoto
AU - Akatsu, Hiroyasu
AU - Li, Feng
AU - Fujita, Hiroko
AU - Nagai, Yasuo
AU - Yamamoto, Takayuki
AU - Okada, Hidechika
AU - Kosaka, Kenji
AU - Sakanaka, Masahiro
AU - Sawada, Tohru
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Recent advances in cDNA microarray technology have made it possible to analyze expression of more than 8000 genes. Using this technology, gene expression in the hippocampus containing neurofibrillary tangle-associated lesions from an Alzheimer's disease (AD) patient was compared with expression in the parietal cortex from the same patient that lacked these lesions. We also compared gene expression using a control brain. The top 20 named genes significantly up-regulated or down-regulated only in the AD brain were determined. The most up-regulated gene proved to be calcineurin Aβ mRNA (CAβ). In situ hybridization histochemistry revealed that CAβ was significantly up-regulated in pyramidal neurons of the hippocampus in the AD brain. RT-PCR analysis revealed that CAβ was up-regulated in the hippocampus from two out of three AD brains while there were no changes in three control brains. Our study suggests that CAβ may play a crucial role in the pathophysiological mechanisms in AD.
AB - Recent advances in cDNA microarray technology have made it possible to analyze expression of more than 8000 genes. Using this technology, gene expression in the hippocampus containing neurofibrillary tangle-associated lesions from an Alzheimer's disease (AD) patient was compared with expression in the parietal cortex from the same patient that lacked these lesions. We also compared gene expression using a control brain. The top 20 named genes significantly up-regulated or down-regulated only in the AD brain were determined. The most up-regulated gene proved to be calcineurin Aβ mRNA (CAβ). In situ hybridization histochemistry revealed that CAβ was significantly up-regulated in pyramidal neurons of the hippocampus in the AD brain. RT-PCR analysis revealed that CAβ was up-regulated in the hippocampus from two out of three AD brains while there were no changes in three control brains. Our study suggests that CAβ may play a crucial role in the pathophysiological mechanisms in AD.
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U2 - 10.1006/bbrc.2001.4968
DO - 10.1006/bbrc.2001.4968
M3 - Article
C2 - 11394878
AN - SCOPUS:18544401772
VL - 284
SP - 310
EP - 316
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -