Up-Regulation of Osteopontin, Chemokines, Adhesion Molecule, and Heat Shock Proteins in 1-Hour Biopsy From Cardiac Death Donor Kidneys

M. Kusaka, Y. Kuroyanagi, T. Mori, H. Sasaki, T. Maruyama, K. Hayakawa, R. Shiroki, Hiroki Kurahashi, K. Hoshinaga

Research output: Contribution to journalArticle

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Abstract

Aims: Since April 1979, 471 kidneys were retrieved from donors after cardiac death (DCD) using an in situ regional cooling technique, with excellent renal function and good long-term graft survival. However, the precise cascade of events following transplantation of DCD kidneys and the influence of ischemia-reperfusion injury remain unclear. In this study, we performed gene expression profiling using 1-hour biopsy samples from DCD kidneys versus those from living sources. Methods: All kidney grafts were procured at our center using an in situ regional cooling technique from DCD. Living donor kidneys (LD) were harvested by open nephrectomy. All graft biopsies were performed 1 hour after reperfusion (DCD n = 8, LD n = 9). We analyzed the expression profile of 20,173 genes. Results: One hundred seventy eight genes were up-regulated (>2-fold difference and DCD/LD > 1.5) and 120 down-regulated (<1/2-fold and LD/DCD > 1.5) in DCD kidneys. Expression of osteopontin (22.5 ± 2.6-fold DCD vs 7.7 ± 1.7 LD; P < .001), chemokines (CCL4 4.4 ± 0.7 vs 2.5 ± 0.3; P < .01), (CCL2 6.0 ± 1.3 vs 2.8 ± 0.5), CXCL1 (9.5 ± 0.4 vs 2.0 ± 0.2), and CXCL2 (16.7 ± 5.3 vs 4.8 ± 1.3; P < .05), adhesion molecule (ICAM-1 4.7 ± 0.7 vs 2.5 ± 0.4; P < .05), and heat shock proteins (HSPA1L 6.7 ± 0.7 vs 1.6 ± 0.3, HSPA1A 17.7 ± 2.6 vs 2.4 ± 0.5, HSPA1B 13.3 ± 0.2 vs 3.0 ± 0.7, HSPA5 6.7 ± 0.8 vs 3.2 ± 0.3, HSPB1 2.9 ± 0.2 vs 1.0 ± 0.1, and HSPH1 19.4 ± 3.0 vs 5.9 ± 1.1; P < .001) were up-regulated in the kidneys from DCD. Conclusion: This report analyzed global gene expression using 1-hour biopsy samples from DCD kidneys. These results may provide new insight into the identification of novel target genes for the development of therapeutic approaches and for determining graft viability of kidneys from DCD.

Original languageEnglish
Pages (from-to)3347-3350
Number of pages4
JournalTransplantation Proceedings
Volume38
Issue number10
DOIs
Publication statusPublished - 01-12-2006

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Osteopontin
Heat-Shock Proteins
Chemokines
Up-Regulation
Tissue Donors
Kidney
Biopsy
Living Donors
Transplants
Chemokine CCL4
Genes
Gene Expression Profiling
Graft Survival
Intercellular Adhesion Molecule-1
Reperfusion Injury
Nephrectomy
Reperfusion

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Kusaka, M. ; Kuroyanagi, Y. ; Mori, T. ; Sasaki, H. ; Maruyama, T. ; Hayakawa, K. ; Shiroki, R. ; Kurahashi, Hiroki ; Hoshinaga, K. / Up-Regulation of Osteopontin, Chemokines, Adhesion Molecule, and Heat Shock Proteins in 1-Hour Biopsy From Cardiac Death Donor Kidneys. In: Transplantation Proceedings. 2006 ; Vol. 38, No. 10. pp. 3347-3350.
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abstract = "Aims: Since April 1979, 471 kidneys were retrieved from donors after cardiac death (DCD) using an in situ regional cooling technique, with excellent renal function and good long-term graft survival. However, the precise cascade of events following transplantation of DCD kidneys and the influence of ischemia-reperfusion injury remain unclear. In this study, we performed gene expression profiling using 1-hour biopsy samples from DCD kidneys versus those from living sources. Methods: All kidney grafts were procured at our center using an in situ regional cooling technique from DCD. Living donor kidneys (LD) were harvested by open nephrectomy. All graft biopsies were performed 1 hour after reperfusion (DCD n = 8, LD n = 9). We analyzed the expression profile of 20,173 genes. Results: One hundred seventy eight genes were up-regulated (>2-fold difference and DCD/LD > 1.5) and 120 down-regulated (<1/2-fold and LD/DCD > 1.5) in DCD kidneys. Expression of osteopontin (22.5 ± 2.6-fold DCD vs 7.7 ± 1.7 LD; P < .001), chemokines (CCL4 4.4 ± 0.7 vs 2.5 ± 0.3; P < .01), (CCL2 6.0 ± 1.3 vs 2.8 ± 0.5), CXCL1 (9.5 ± 0.4 vs 2.0 ± 0.2), and CXCL2 (16.7 ± 5.3 vs 4.8 ± 1.3; P < .05), adhesion molecule (ICAM-1 4.7 ± 0.7 vs 2.5 ± 0.4; P < .05), and heat shock proteins (HSPA1L 6.7 ± 0.7 vs 1.6 ± 0.3, HSPA1A 17.7 ± 2.6 vs 2.4 ± 0.5, HSPA1B 13.3 ± 0.2 vs 3.0 ± 0.7, HSPA5 6.7 ± 0.8 vs 3.2 ± 0.3, HSPB1 2.9 ± 0.2 vs 1.0 ± 0.1, and HSPH1 19.4 ± 3.0 vs 5.9 ± 1.1; P < .001) were up-regulated in the kidneys from DCD. Conclusion: This report analyzed global gene expression using 1-hour biopsy samples from DCD kidneys. These results may provide new insight into the identification of novel target genes for the development of therapeutic approaches and for determining graft viability of kidneys from DCD.",
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Up-Regulation of Osteopontin, Chemokines, Adhesion Molecule, and Heat Shock Proteins in 1-Hour Biopsy From Cardiac Death Donor Kidneys. / Kusaka, M.; Kuroyanagi, Y.; Mori, T.; Sasaki, H.; Maruyama, T.; Hayakawa, K.; Shiroki, R.; Kurahashi, Hiroki; Hoshinaga, K.

In: Transplantation Proceedings, Vol. 38, No. 10, 01.12.2006, p. 3347-3350.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Up-Regulation of Osteopontin, Chemokines, Adhesion Molecule, and Heat Shock Proteins in 1-Hour Biopsy From Cardiac Death Donor Kidneys

AU - Kusaka, M.

AU - Kuroyanagi, Y.

AU - Mori, T.

AU - Sasaki, H.

AU - Maruyama, T.

AU - Hayakawa, K.

AU - Shiroki, R.

AU - Kurahashi, Hiroki

AU - Hoshinaga, K.

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Aims: Since April 1979, 471 kidneys were retrieved from donors after cardiac death (DCD) using an in situ regional cooling technique, with excellent renal function and good long-term graft survival. However, the precise cascade of events following transplantation of DCD kidneys and the influence of ischemia-reperfusion injury remain unclear. In this study, we performed gene expression profiling using 1-hour biopsy samples from DCD kidneys versus those from living sources. Methods: All kidney grafts were procured at our center using an in situ regional cooling technique from DCD. Living donor kidneys (LD) were harvested by open nephrectomy. All graft biopsies were performed 1 hour after reperfusion (DCD n = 8, LD n = 9). We analyzed the expression profile of 20,173 genes. Results: One hundred seventy eight genes were up-regulated (>2-fold difference and DCD/LD > 1.5) and 120 down-regulated (<1/2-fold and LD/DCD > 1.5) in DCD kidneys. Expression of osteopontin (22.5 ± 2.6-fold DCD vs 7.7 ± 1.7 LD; P < .001), chemokines (CCL4 4.4 ± 0.7 vs 2.5 ± 0.3; P < .01), (CCL2 6.0 ± 1.3 vs 2.8 ± 0.5), CXCL1 (9.5 ± 0.4 vs 2.0 ± 0.2), and CXCL2 (16.7 ± 5.3 vs 4.8 ± 1.3; P < .05), adhesion molecule (ICAM-1 4.7 ± 0.7 vs 2.5 ± 0.4; P < .05), and heat shock proteins (HSPA1L 6.7 ± 0.7 vs 1.6 ± 0.3, HSPA1A 17.7 ± 2.6 vs 2.4 ± 0.5, HSPA1B 13.3 ± 0.2 vs 3.0 ± 0.7, HSPA5 6.7 ± 0.8 vs 3.2 ± 0.3, HSPB1 2.9 ± 0.2 vs 1.0 ± 0.1, and HSPH1 19.4 ± 3.0 vs 5.9 ± 1.1; P < .001) were up-regulated in the kidneys from DCD. Conclusion: This report analyzed global gene expression using 1-hour biopsy samples from DCD kidneys. These results may provide new insight into the identification of novel target genes for the development of therapeutic approaches and for determining graft viability of kidneys from DCD.

AB - Aims: Since April 1979, 471 kidneys were retrieved from donors after cardiac death (DCD) using an in situ regional cooling technique, with excellent renal function and good long-term graft survival. However, the precise cascade of events following transplantation of DCD kidneys and the influence of ischemia-reperfusion injury remain unclear. In this study, we performed gene expression profiling using 1-hour biopsy samples from DCD kidneys versus those from living sources. Methods: All kidney grafts were procured at our center using an in situ regional cooling technique from DCD. Living donor kidneys (LD) were harvested by open nephrectomy. All graft biopsies were performed 1 hour after reperfusion (DCD n = 8, LD n = 9). We analyzed the expression profile of 20,173 genes. Results: One hundred seventy eight genes were up-regulated (>2-fold difference and DCD/LD > 1.5) and 120 down-regulated (<1/2-fold and LD/DCD > 1.5) in DCD kidneys. Expression of osteopontin (22.5 ± 2.6-fold DCD vs 7.7 ± 1.7 LD; P < .001), chemokines (CCL4 4.4 ± 0.7 vs 2.5 ± 0.3; P < .01), (CCL2 6.0 ± 1.3 vs 2.8 ± 0.5), CXCL1 (9.5 ± 0.4 vs 2.0 ± 0.2), and CXCL2 (16.7 ± 5.3 vs 4.8 ± 1.3; P < .05), adhesion molecule (ICAM-1 4.7 ± 0.7 vs 2.5 ± 0.4; P < .05), and heat shock proteins (HSPA1L 6.7 ± 0.7 vs 1.6 ± 0.3, HSPA1A 17.7 ± 2.6 vs 2.4 ± 0.5, HSPA1B 13.3 ± 0.2 vs 3.0 ± 0.7, HSPA5 6.7 ± 0.8 vs 3.2 ± 0.3, HSPB1 2.9 ± 0.2 vs 1.0 ± 0.1, and HSPH1 19.4 ± 3.0 vs 5.9 ± 1.1; P < .001) were up-regulated in the kidneys from DCD. Conclusion: This report analyzed global gene expression using 1-hour biopsy samples from DCD kidneys. These results may provide new insight into the identification of novel target genes for the development of therapeutic approaches and for determining graft viability of kidneys from DCD.

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