Up-regulation of telomerase in primary cultured rat hepatocytes

Katsura Nozawa, Yasuhiro Kurumiya, Akiko Yamamoto, Yuka Isobe, Motoshi Suzuki, Shonen Yoshida

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells, we examined telomerase activity in primary cultured rat hepatocytes. During culture under serum-free conditions, rat hepatocytes rapidly lose the ability of organ-specific expression of serum albumin, apolipoprotein A-I, and hepatocyte nuclear factor 4, and the capacity for cytochrome P-450 induction by xenobiotics. The telomerase activity was found to be concomitantly increased about 2.5-fold at 48 h and 3-fold at 72 h. Northern blot and RT-PCR analyses with primary cultured hepatocytes revealed the associated accumulation of rat telomerase RNA subunits (TR), and the mRNAs for a telomerase reverse transcriptase (TERT) and a telomerase-associated protein (TEP1). The activity of hepatocyte telomerase, which was elevated during the primary culture, increased further when the cells were stimulated with hepatocyte growth factor. In this case, however, the levels of TR, TERT, and TEP1 mRNA did not show any detectable changes.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalJournal of Biochemistry
Volume126
Issue number2
DOIs
Publication statusPublished - 01-01-1999
Externally publishedYes

Fingerprint

Telomerase
Rats
Hepatocytes
Up-Regulation
Hepatocyte Nuclear Factor 4
Messenger RNA
Hepatocyte Growth Factor
RNA-Directed DNA Polymerase
Apolipoprotein A-I
Xenobiotics
Serum Albumin
Northern Blotting
Liver
Cytochrome P-450 Enzyme System
Rodentia
Maintenance
Polymerase Chain Reaction
DNA
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Nozawa, Katsura ; Kurumiya, Yasuhiro ; Yamamoto, Akiko ; Isobe, Yuka ; Suzuki, Motoshi ; Yoshida, Shonen. / Up-regulation of telomerase in primary cultured rat hepatocytes. In: Journal of Biochemistry. 1999 ; Vol. 126, No. 2. pp. 361-367.
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Nozawa, K, Kurumiya, Y, Yamamoto, A, Isobe, Y, Suzuki, M & Yoshida, S 1999, 'Up-regulation of telomerase in primary cultured rat hepatocytes', Journal of Biochemistry, vol. 126, no. 2, pp. 361-367. https://doi.org/10.1093/oxfordjournals.jbchem.a022458

Up-regulation of telomerase in primary cultured rat hepatocytes. / Nozawa, Katsura; Kurumiya, Yasuhiro; Yamamoto, Akiko; Isobe, Yuka; Suzuki, Motoshi; Yoshida, Shonen.

In: Journal of Biochemistry, Vol. 126, No. 2, 01.01.1999, p. 361-367.

Research output: Contribution to journalArticle

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AU - Nozawa, Katsura

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